F Lapicque, P Netter, C Monot, B Bannwarth, M S Maknassi, R J Royer
{"title":"用平衡透析法测定药物的蛋白质结合。渗透压对体积位移的影响[j]。","authors":"F Lapicque, P Netter, C Monot, B Bannwarth, M S Maknassi, R J Royer","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>By the determination of the free concentration of a drug using the equilibrium dialysis method, a volume shift could be observed, inducing a dilution of the protein medium. The value of this volume shift varies with the plasmatic sample and could produce a misdetermination of the free fraction which could be important, essentially for the drugs strongly bound to proteins.</p>","PeriodicalId":14817,"journal":{"name":"Journal de pharmacologie","volume":"17 3","pages":"295-300"},"PeriodicalIF":0.0000,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Determination of protein binding of drugs using equilibrium dialysis. Influence of volume displacement caused by osmotic pressure].\",\"authors\":\"F Lapicque, P Netter, C Monot, B Bannwarth, M S Maknassi, R J Royer\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>By the determination of the free concentration of a drug using the equilibrium dialysis method, a volume shift could be observed, inducing a dilution of the protein medium. The value of this volume shift varies with the plasmatic sample and could produce a misdetermination of the free fraction which could be important, essentially for the drugs strongly bound to proteins.</p>\",\"PeriodicalId\":14817,\"journal\":{\"name\":\"Journal de pharmacologie\",\"volume\":\"17 3\",\"pages\":\"295-300\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1986-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal de pharmacologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal de pharmacologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Determination of protein binding of drugs using equilibrium dialysis. Influence of volume displacement caused by osmotic pressure].
By the determination of the free concentration of a drug using the equilibrium dialysis method, a volume shift could be observed, inducing a dilution of the protein medium. The value of this volume shift varies with the plasmatic sample and could produce a misdetermination of the free fraction which could be important, essentially for the drugs strongly bound to proteins.