钙诱导细胞大小脂质体与单层膜在不同渗透压溶液中的融合动力学。

N Stoicheva, I Tsoneva, D S Dimitrov, I Panaiotov
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引用次数: 3

摘要

研究了亚相中渗透压、钙浓度和细胞大小对磷脂单分子膜表面张力的影响。磷脂酸氯仿溶液在空气/水溶液界面上扩散单层膜。磷脂酸/卵磷脂(1:2)的混合物在水中或0.1 M蔗糖水溶液中形成平均直径为3微米的脂质体。对于该系统,钙离子的临界浓度可以使单层表面张力大幅降低。阈值钙浓度取决于亚期蔗糖浓度。无蔗糖时,阈值钙浓度为8 mM,而等渗蔗糖溶液(0.1/0.1 M in/out of lipo质体)的阈值钙浓度为14 mM。当脂质体膜上蔗糖浓度差0.02 M时,阈值钙浓度急剧上升至28 mM CaCl2,当阈值钙浓度差进一步增加至0.04 M、0.06 M和0.08 M时,阈值钙浓度分别下降至26 mM、19 mM和18 mM。在30 mM CaCl2和不同蔗糖浓度的亚相溶液中,测量了单层表面张力下降的速率作为时间的函数。初始速率起初随渗透压的增加而降低,之后又增加。最小值出现在脂质体膜上的蔗糖浓度梯度为0.02 M时,即在单分子膜表面张力大幅降低所需的最大阈值钙浓度点。这些事实可以用最近的薄膜黏附、不稳定和融合动力学理论来解释。
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Kinetics of calcium-induced fusion of cell-size liposomes with monolayers in solutions of different osmolarity.

The effects of osmolarity, calcium concentration and cell-size liposomes in the subphase on the surface tension of phospholipid monolayers were investigated. The monolayers were spread from chloroform solutions of phosphatidic acid at air/water solution interface. The liposomes (of average diameter 3 micron) were formed from phosphatidic acid/egg lecithin (1:2) mixtures in water or 0.1 M water solutions of sucrose. For this system there were critical concentrations of calcium ions to produce a large reduction of the monolayer surface tension. The threshold calcium concentrations depended upon the sucrose concentration in the subphase. Without sucrose the threshold calcium concentration was 8 mM, while for isoosmotic sucrose solutions (0.1/0.1 M in/out of liposome) it was 14 mM. It sharply increased to 28 mM CaCl2 at sucrose concentration difference across the liposome membrane 0.02 M and decreased to 26 mM, 19 mM, and 18 mM with further increase of that difference to 0.04 M, 0.06 M, and 0.08 M, respectively. The rate of monolayer surface tension decrease was measured as a function of time at 30 mM CaCl2 and different sucrose concentrations in the subphase solution. The initial rates at first decreased with increasing the osmotic pressure and after that they increased. The minimum occurred at sucrose concentration gradient across the liposome membrane 0.02 M, i.e., at the point of maximum threshold calcium concentration required for large decrease of the monolayer surface tension. These facts may be explained by recent theories of dynamics of adhesion, instability and fusion of membranes modeled as thin films.

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