人抗甲状腺球蛋白抗体放射免疫测定。1 .晒黑细胞血凝与双抗体技术的关系。

Hormones Pub Date : 1972-01-01 DOI:10.1159/000178252
G B Salabé, S Fontana, M Andreoli
{"title":"人抗甲状腺球蛋白抗体放射免疫测定。1 .晒黑细胞血凝与双抗体技术的关系。","authors":"G B Salabé, S Fontana, M Andreoli","doi":"10.1159/000178252","DOIUrl":null,"url":null,"abstract":"The present study describes a double antibody technique to evaluate antithyroglobulin autoantibodies in human serum. Rabbit anti-human γG is used to precipitate the immunocomplexes between labelled thyroglobulin and autoantibodies. Thyroglobulin has been labelled either in vivo, or chemically by electrolysis of iodide, a procedure which also produced substantial dissociation of the protein. The double antibody technique was compared with haemagglutination of sheep red blood cells coated with human thyroglobulin. A good correlation was established between the tanned red cell haemagglutination titre and the double antibody technique when ‘ in vivo’ labelled thyroglobulin was employed. The use of chemically iodinated thyroglobulin, purified after labelling, increased the sensitivity of the test 100 fold so that 38% of sera from patients with thyroid diseases having negative haemagglutination titres precipitate between 15% and 65% of the labelled antigen. The sensitivity and simplicity of this method provide a useful tool in clinical as well as in experimental work.","PeriodicalId":13017,"journal":{"name":"Hormones","volume":"3 1","pages":"1-13"},"PeriodicalIF":0.0000,"publicationDate":"1972-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000178252","citationCount":"38","resultStr":"{\"title\":\"Radioimmunoassay for human antithyroglobulin antibodies. I. The relationship between tanned cell haemagglutination and a double antibody technique.\",\"authors\":\"G B Salabé, S Fontana, M Andreoli\",\"doi\":\"10.1159/000178252\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study describes a double antibody technique to evaluate antithyroglobulin autoantibodies in human serum. Rabbit anti-human γG is used to precipitate the immunocomplexes between labelled thyroglobulin and autoantibodies. Thyroglobulin has been labelled either in vivo, or chemically by electrolysis of iodide, a procedure which also produced substantial dissociation of the protein. The double antibody technique was compared with haemagglutination of sheep red blood cells coated with human thyroglobulin. A good correlation was established between the tanned red cell haemagglutination titre and the double antibody technique when ‘ in vivo’ labelled thyroglobulin was employed. The use of chemically iodinated thyroglobulin, purified after labelling, increased the sensitivity of the test 100 fold so that 38% of sera from patients with thyroid diseases having negative haemagglutination titres precipitate between 15% and 65% of the labelled antigen. The sensitivity and simplicity of this method provide a useful tool in clinical as well as in experimental work.\",\"PeriodicalId\":13017,\"journal\":{\"name\":\"Hormones\",\"volume\":\"3 1\",\"pages\":\"1-13\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1972-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1159/000178252\",\"citationCount\":\"38\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hormones\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1159/000178252\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hormones","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000178252","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 38
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Radioimmunoassay for human antithyroglobulin antibodies. I. The relationship between tanned cell haemagglutination and a double antibody technique.
The present study describes a double antibody technique to evaluate antithyroglobulin autoantibodies in human serum. Rabbit anti-human γG is used to precipitate the immunocomplexes between labelled thyroglobulin and autoantibodies. Thyroglobulin has been labelled either in vivo, or chemically by electrolysis of iodide, a procedure which also produced substantial dissociation of the protein. The double antibody technique was compared with haemagglutination of sheep red blood cells coated with human thyroglobulin. A good correlation was established between the tanned red cell haemagglutination titre and the double antibody technique when ‘ in vivo’ labelled thyroglobulin was employed. The use of chemically iodinated thyroglobulin, purified after labelling, increased the sensitivity of the test 100 fold so that 38% of sera from patients with thyroid diseases having negative haemagglutination titres precipitate between 15% and 65% of the labelled antigen. The sensitivity and simplicity of this method provide a useful tool in clinical as well as in experimental work.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
An evidence-based assessment of the nutritional recommendations for the prevention of diabetes mellitus CUL1 exacerbates glucocorticoid-induced osteoporosis by enhancing ASAP1 ubiquitination Joint effects of tobacco smoke exposure and heavy metals on serum sex hormones in adult males Spirulina supplementation and circuit resistance training (CRT) reduce serum asprosin and appetite and improve energy balance in men with obesity and overweight A schedule for tapering glucocorticoid treatment in patients with severe SARS-CoV 2 infection can prevent acute adrenal insufficiency in the geriatric population.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1