{"title":"鳖心脏线粒体腺苷三磷酸酶活性的研究","authors":"A.J. Rotermund , C.A. Privitera","doi":"10.1016/0010-406X(70)90953-9","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. ATPase activity has been estimated in heart mitochondria of the fresh-water turtle, <em>Chrysemys picta</em>.</p></span></li><li><span>2.</span><span><p>2. This activity is Mg<sup>2+</sup>-dependent and stimulated by the presence of DNP.</p></span></li><li><span>3.</span><span><p>3. The pH optima of ATPase activities are 8·5 with Mg<sup>2+</sup> ions, and 7·4 in the presence of DNP.</p></span></li><li><span>4.</span><span><p>4. Differential responses to inhibitors are evident in the presence of added Mg<sup>2+</sup> ions or DNP, with DNP-stimulated activities being sensitive to a wider spectrum of inhibitors and to the aging process.</p></span></li><li><span>5.</span><span><p>5. Bovine serum albumin, but not EDTA, effectively preserves DNP-stimulated ATPase activity during aging. A causal relationship with fatty acids is implied.</p></span></li><li><span>6.</span><span><p>6. These data may be used to support either a one or a two enzyme hypothesis, with the latter being favored.</p></span></li></ul></div>","PeriodicalId":78189,"journal":{"name":"Comparative biochemistry and physiology","volume":"37 1","pages":"Pages 1-22"},"PeriodicalIF":0.0000,"publicationDate":"1970-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0010-406X(70)90953-9","citationCount":"4","resultStr":"{\"title\":\"Adenosine triphosphatase activity of heart mitochondria of the turtle, Chrysemys picta\",\"authors\":\"A.J. Rotermund , C.A. Privitera\",\"doi\":\"10.1016/0010-406X(70)90953-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. ATPase activity has been estimated in heart mitochondria of the fresh-water turtle, <em>Chrysemys picta</em>.</p></span></li><li><span>2.</span><span><p>2. This activity is Mg<sup>2+</sup>-dependent and stimulated by the presence of DNP.</p></span></li><li><span>3.</span><span><p>3. The pH optima of ATPase activities are 8·5 with Mg<sup>2+</sup> ions, and 7·4 in the presence of DNP.</p></span></li><li><span>4.</span><span><p>4. Differential responses to inhibitors are evident in the presence of added Mg<sup>2+</sup> ions or DNP, with DNP-stimulated activities being sensitive to a wider spectrum of inhibitors and to the aging process.</p></span></li><li><span>5.</span><span><p>5. Bovine serum albumin, but not EDTA, effectively preserves DNP-stimulated ATPase activity during aging. A causal relationship with fatty acids is implied.</p></span></li><li><span>6.</span><span><p>6. These data may be used to support either a one or a two enzyme hypothesis, with the latter being favored.</p></span></li></ul></div>\",\"PeriodicalId\":78189,\"journal\":{\"name\":\"Comparative biochemistry and physiology\",\"volume\":\"37 1\",\"pages\":\"Pages 1-22\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1970-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0010-406X(70)90953-9\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative biochemistry and physiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0010406X70909539\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0010406X70909539","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Adenosine triphosphatase activity of heart mitochondria of the turtle, Chrysemys picta
1.
1. ATPase activity has been estimated in heart mitochondria of the fresh-water turtle, Chrysemys picta.
2.
2. This activity is Mg2+-dependent and stimulated by the presence of DNP.
3.
3. The pH optima of ATPase activities are 8·5 with Mg2+ ions, and 7·4 in the presence of DNP.
4.
4. Differential responses to inhibitors are evident in the presence of added Mg2+ ions or DNP, with DNP-stimulated activities being sensitive to a wider spectrum of inhibitors and to the aging process.
5.
5. Bovine serum albumin, but not EDTA, effectively preserves DNP-stimulated ATPase activity during aging. A causal relationship with fatty acids is implied.
6.
6. These data may be used to support either a one or a two enzyme hypothesis, with the latter being favored.
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