The production of hyaluronate by spontaneously established cell lines and viral transformed lines of fibroblastic origin

Human diploid fibroblast strains and established mouse fibroblast lines continue to synthesize hyaluronate over many cell generations in culture. However, this property may be lost in the course of many years of serial culture of established lines as it is no longer detectable in three well-known mouse lines of fibroblastic origin, including L-cells. Transformation of the established mouse fibroblast line 3T3 by SV₄₀ or polyoma virus and of human diploid fibroblast strains by SV₄₀ leads in each case to a marked diminution in the rate of hyaluronate synthesis.