{"title":"无果是性别差异拼接,对家蚕的求偶行为和发育具有重要意义","authors":"Masumi Ueno , Masami Nakata, Yoshiki Kaneko, Masafumi Iwami, Seika Takayanagi-Kiya, Taketoshi Kiya","doi":"10.1016/j.ibmb.2023.103989","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Sexual dimorphisms of the brain play essential roles in successful reproduction. </span>Silkmoth </span><em>Bombyx mori</em><span> exhibits extensive sexual differences in sexual behavior, as well as their morphology. Although the neural circuits that transmit information about sex pheromone in the male brain are extensively analyzed, the molecular mechanisms that regulate their development are still elusive. In the present study, we focused on the silkmoth ortholog of </span><em>fruitless</em> (<em>fru</em>) as a candidate gene that regulates sexual dimorphisms of the brain. <em>fru</em> transcripts were expressed from multiple promoters in various tissues, and brain-specific transcripts were sex-specifically spliced, in a manner similar to <em>Drosophila</em>. Interestingly, <em>fru</em><span> was highly expressed in the adult female brain and the male larval testis. Analysis of CRISPR/Cas9-mediated </span><em>fru</em> knockout strains revealed that <em>fru</em> plays important roles in survival during late larval and pupal stages, testis development, and adult sexual behavior. <em>fru</em> mutant males exhibited highly reduced levels of courtship and low copulation rate, indicating that <em>fru</em> plays significant roles in the sexual behavior of silkmoths, although it is not absolutely necessary for copulation. In the <em>fru</em><span> mutant males, sexually dimorphic pattern of the odorant receptor expression was impaired, possibly causing the defects in courtship behavior. These results provide important clues to elucidate the development of sexual dimorphisms of silkmoth brains, as well as the evolution of </span><em>fruitless</em> gene in insects.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"fruitless is sex-differentially spliced and is important for the courtship behavior and development of silkmoth Bombyx mori\",\"authors\":\"Masumi Ueno , Masami Nakata, Yoshiki Kaneko, Masafumi Iwami, Seika Takayanagi-Kiya, Taketoshi Kiya\",\"doi\":\"10.1016/j.ibmb.2023.103989\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>Sexual dimorphisms of the brain play essential roles in successful reproduction. </span>Silkmoth </span><em>Bombyx mori</em><span> exhibits extensive sexual differences in sexual behavior, as well as their morphology. Although the neural circuits that transmit information about sex pheromone in the male brain are extensively analyzed, the molecular mechanisms that regulate their development are still elusive. In the present study, we focused on the silkmoth ortholog of </span><em>fruitless</em> (<em>fru</em>) as a candidate gene that regulates sexual dimorphisms of the brain. <em>fru</em> transcripts were expressed from multiple promoters in various tissues, and brain-specific transcripts were sex-specifically spliced, in a manner similar to <em>Drosophila</em>. Interestingly, <em>fru</em><span> was highly expressed in the adult female brain and the male larval testis. Analysis of CRISPR/Cas9-mediated </span><em>fru</em> knockout strains revealed that <em>fru</em> plays important roles in survival during late larval and pupal stages, testis development, and adult sexual behavior. <em>fru</em> mutant males exhibited highly reduced levels of courtship and low copulation rate, indicating that <em>fru</em> plays significant roles in the sexual behavior of silkmoths, although it is not absolutely necessary for copulation. In the <em>fru</em><span> mutant males, sexually dimorphic pattern of the odorant receptor expression was impaired, possibly causing the defects in courtship behavior. These results provide important clues to elucidate the development of sexual dimorphisms of silkmoth brains, as well as the evolution of </span><em>fruitless</em> gene in insects.</p></div>\",\"PeriodicalId\":330,\"journal\":{\"name\":\"Insect Biochemistry and Molecular Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2023-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0965174823000838\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0965174823000838","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
fruitless is sex-differentially spliced and is important for the courtship behavior and development of silkmoth Bombyx mori
Sexual dimorphisms of the brain play essential roles in successful reproduction. Silkmoth Bombyx mori exhibits extensive sexual differences in sexual behavior, as well as their morphology. Although the neural circuits that transmit information about sex pheromone in the male brain are extensively analyzed, the molecular mechanisms that regulate their development are still elusive. In the present study, we focused on the silkmoth ortholog of fruitless (fru) as a candidate gene that regulates sexual dimorphisms of the brain. fru transcripts were expressed from multiple promoters in various tissues, and brain-specific transcripts were sex-specifically spliced, in a manner similar to Drosophila. Interestingly, fru was highly expressed in the adult female brain and the male larval testis. Analysis of CRISPR/Cas9-mediated fru knockout strains revealed that fru plays important roles in survival during late larval and pupal stages, testis development, and adult sexual behavior. fru mutant males exhibited highly reduced levels of courtship and low copulation rate, indicating that fru plays significant roles in the sexual behavior of silkmoths, although it is not absolutely necessary for copulation. In the fru mutant males, sexually dimorphic pattern of the odorant receptor expression was impaired, possibly causing the defects in courtship behavior. These results provide important clues to elucidate the development of sexual dimorphisms of silkmoth brains, as well as the evolution of fruitless gene in insects.
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.