{"title":"半透明Nitella translucens对磷酸盐的活性吸收","authors":"F.A. Smith","doi":"10.1016/0926-6585(66)90040-9","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Using <sup>32</sup>P as a tracer, phosphate uptake into the giant cells of <em>Nitella translucens</em> has been shown to be a strictly active process. The uptake is light-enhanced and requires a supply of ATP. When non-cyclic electron flow is prevented, the light-dependent process is supported by cyclic photophosphorylation.</p></span></li><li><span>2.</span><span><p>2. As it is already known that active Cl<sup>−</sup> uptake into <em>Nitella translucens</em> is not dependent on ATP but is linked to electron-transfer reactions, there can be no common mechanism for anion transport in these cells.</p></span></li><li><span>3.</span><span><p>3. Measurements of the relative amounts of <sup>32</sup>P in the cytoplasm and vacuole have shown that the cytoplasm contains nearly all the organic phosphate, while a large pool of inorganic phosphate may be formed in the vacuole.</p></span></li></ul></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"126 1","pages":"Pages 94-99"},"PeriodicalIF":0.0000,"publicationDate":"1966-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90040-9","citationCount":"62","resultStr":"{\"title\":\"Active phosphate uptake by Nitella translucens\",\"authors\":\"F.A. Smith\",\"doi\":\"10.1016/0926-6585(66)90040-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Using <sup>32</sup>P as a tracer, phosphate uptake into the giant cells of <em>Nitella translucens</em> has been shown to be a strictly active process. The uptake is light-enhanced and requires a supply of ATP. When non-cyclic electron flow is prevented, the light-dependent process is supported by cyclic photophosphorylation.</p></span></li><li><span>2.</span><span><p>2. As it is already known that active Cl<sup>−</sup> uptake into <em>Nitella translucens</em> is not dependent on ATP but is linked to electron-transfer reactions, there can be no common mechanism for anion transport in these cells.</p></span></li><li><span>3.</span><span><p>3. Measurements of the relative amounts of <sup>32</sup>P in the cytoplasm and vacuole have shown that the cytoplasm contains nearly all the organic phosphate, while a large pool of inorganic phosphate may be formed in the vacuole.</p></span></li></ul></div>\",\"PeriodicalId\":100158,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"volume\":\"126 1\",\"pages\":\"Pages 94-99\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6585(66)90040-9\",\"citationCount\":\"62\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926658566900409\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926658566900409","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
1. Using 32P as a tracer, phosphate uptake into the giant cells of Nitella translucens has been shown to be a strictly active process. The uptake is light-enhanced and requires a supply of ATP. When non-cyclic electron flow is prevented, the light-dependent process is supported by cyclic photophosphorylation.
2.
2. As it is already known that active Cl− uptake into Nitella translucens is not dependent on ATP but is linked to electron-transfer reactions, there can be no common mechanism for anion transport in these cells.
3.
3. Measurements of the relative amounts of 32P in the cytoplasm and vacuole have shown that the cytoplasm contains nearly all the organic phosphate, while a large pool of inorganic phosphate may be formed in the vacuole.