{"title":"[利用蛋白膜进行定量细胞光度测定的模型实验(作者译)]。","authors":"G Desoye, E Schauenstein","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The usefulness of protein films denaturated and fixed with ethanol/ether as cytochemical model systems was investigated. Kinetics of staining with amido black 10B were analyzed as well as the spectroscopic properties of the protein-dye complex. The absorption coefficient of the complex was estimated. Data obtained so far are compared with both stained cells and the protein-dye complex in solution. Discussion focuses on the influence of fixation and the possibilities to use the films as systems for calibrating cytochemical stainings.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 1","pages":"9-16"},"PeriodicalIF":0.0000,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Model experiments for quantitative cytophotometry using protein films (author's transl)].\",\"authors\":\"G Desoye, E Schauenstein\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The usefulness of protein films denaturated and fixed with ethanol/ether as cytochemical model systems was investigated. Kinetics of staining with amido black 10B were analyzed as well as the spectroscopic properties of the protein-dye complex. The absorption coefficient of the complex was estimated. Data obtained so far are compared with both stained cells and the protein-dye complex in solution. Discussion focuses on the influence of fixation and the possibilities to use the films as systems for calibrating cytochemical stainings.</p>\",\"PeriodicalId\":76158,\"journal\":{\"name\":\"Microscopica acta\",\"volume\":\"84 1\",\"pages\":\"9-16\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microscopica acta\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopica acta","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Model experiments for quantitative cytophotometry using protein films (author's transl)].
The usefulness of protein films denaturated and fixed with ethanol/ether as cytochemical model systems was investigated. Kinetics of staining with amido black 10B were analyzed as well as the spectroscopic properties of the protein-dye complex. The absorption coefficient of the complex was estimated. Data obtained so far are compared with both stained cells and the protein-dye complex in solution. Discussion focuses on the influence of fixation and the possibilities to use the films as systems for calibrating cytochemical stainings.
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