Effect of protein aggregation on murine splenic prostaglandin F2α levels

Intravenous injection of deaggregated human gamma globulin and deaggregated keyhole limpet hemocyanin into C57B1/6 mice does not result in an increase in splenic prostaglandin F_2α. However, when aggregated human gamma globulin or aggregated keyhole limpet hemoeyapin were injected, significant increases in splenic PGF_2α were observed. Mice which had been rendered immunologically tolerant to human gamma globulin (HGG) also demonstrated increases in splenic PGF_2α and PGE₂ 5 min after boosting with aggregated HGG with no increases in antibody titer against HGG. These results imply that the property of protein aggregation will engender increases in splenic prostaglandin levels and that these increases don't appear to affect the tolerant state.