用淀粉酶-磷酸化酶反应器以葡萄糖-磷酸为原料连续生产低聚麦芽糖。

Journal of applied biochemistry Pub Date : 1983-12-01
H Nakatani, A Tanaka, K Hiromi
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引用次数: 0

摘要

利用超滤细胞,通过α -淀粉酶或β -淀粉酶和磷酸化酶的共同作用,实现了麦芽糖和其他低聚糖混合物的连续生产。对于-淀粉酶-磷酸化酶系统,稳定生产麦芽糖,直到-淀粉酶失活。对于α -淀粉酶-磷酸化酶系统,低聚糖的稳定生产难以维持,这可能是由于α -淀粉酶的随机裂解破坏了底物糖原。
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Continuous production of maltooligosaccharides from glucose 1-phosphate by amylase-phosphorylase reactor.

Using an ultrafiltration cell, the continuous production of maltose and a mixture of other oligosaccharides was achieved by the combined action of alpha- or beta-amylase and phosphorylase. For the beta-amylase-phosphorylase system, a steady production of maltose was obtained until the beta-amylase was inactivated. For the alpha-amylase-phosphorylase system, it was difficult to maintain a stable production of oligosaccharides, probably due to the destruction of substrate glycogen by random cleavage by alpha-amylase.

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