J C Stockert, R Armas-Portela, O D Colman, J M Ferrer, A Tato
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Oxidized p-phenylenediamine staining of epoxy resin sections.
Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable electron opacity in PPD positive structures.
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