胃肠道菌群对自然杀伤细胞活性的影响。

K F Bartizal, C Salkowski, E Balish
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摘要

这些研究表明,消化道微生物可显著增强BALB/c小鼠脾细胞对51cr标记的YAC-1细胞的天然细胞毒性。无菌BALB/c小鼠的脾脏细胞,无论是健胸腺、胸腺或非裸背景(+/+),对YAC-1细胞具有天然细胞介导的细胞毒性。肠道定殖与少数(菌群定义)或许多(复杂的菌群常规)微生物显著增强天然细胞介导的胸腺和胸腺小鼠的细胞毒性。相反,用纯培养的白色念珠菌或用念珠菌和芽孢杆菌定殖胸腺和无菌小鼠的消化道,与无菌水平相比,并没有提高自然细胞介导的细胞毒活性。无菌胸腺小鼠脾细胞的细胞毒性明显高于未携带裸基因(即+/+)的无菌BALB/c小鼠脾细胞。在无菌化或非共生状态下,胸腺型(nu/nu)和杂合型(+/nu)小鼠的自然杀伤细胞活性无明显差异;然而,胸腺(nu/nu)菌群定义或常规小鼠脾细胞对YAC-1细胞的细胞毒性明显高于来自菌群定义或常规杂合(+/nu)窝鼠的脾细胞。在三种不同的效应靶比下,来自BALB/c小鼠胸腺(nu/nu)和复杂微生物菌群定植(即,常规)的脾脏细胞对YAC-1细胞具有最高的细胞毒性百分比。这些研究表明,肠道菌群可以改变小鼠自然细胞介导的细胞毒性。
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The influence of a gastrointestinal microflora on natural killer cell activity.

These studies demonstrate that the natural cytotoxicity of BALB/c mouse spleen cells for 51Cr-labeled YAC-1 cells can be significantly enhanced by microorganisms in the alimentary tract. Spleen cells from germfree BALB/c mice, euthymic, athymic, or non-nude background (+/+), had natural cell-mediated cytotoxicity for YAC-1 cells. Intestinal colonization with a few (flora-defined) or many (complex flora-conventionalized) microorganisms significantly enhanced natural cell-mediated cytotoxicity of athymic and euthymic mice over their germfree counterparts. Conversely, colonization of the alimentary tract of athymic and euthymic germfree mice with a pure culture of Candida albicans or colonization with Candida and a Bacillus sp. did not enhance natural cell-mediated cytotoxic activity over germfree levels. Spleen cells from germfree athymic mice were significantly more cytotoxic than spleen cells from germfree BALB/c mice that did not carry the nude gene (ie, +/+). In the germfree or gnotobiotic state, no difference in natural killer cell activity was evident between athymic (nu/nu) and heterozygous (+/nu) littermate mice; however, athymic (nu/nu) flora-defined or conventionalized mouse spleen cells were significantly more cytotoxic for YAC-1 cells than splenocytes from flora-defined or conventionalized heterozygous (+/nu) littermates. Spleen cells from BALB/c mice that were athymic (nu/nu) and colonized with a complex microbial flora (ie, conventionalized) had the highest percentage of cytotoxicity, at three different effector to target ratios, for YAC-1 cells. These studies indicate that the intestinal microflora can alter murine natural cell-mediated cytotoxicity.

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