人甲状旁腺激素基因(PTH)位于11号染色体短臂上。

Somatic Cell Genetics Pub Date : 1983-09-01 DOI:10.1007/BF01574261

S L Naylor, A Y Sakaguchi, P Szoka, G N Hendy, H M Kronenberg, A Rich, T B Shows

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引用次数: 79

摘要

利用人鼠杂交和细胞杂交DNA的Southern过滤杂交技术，对人类甲状旁腺激素(PTH)基因进行了染色体定位。一个含有人PTH cDNA插入片段(pPTHm122)的重组DNA探针与用EcoRI酶切割的3.7 kb的人DNA片段杂交。通过将体细胞杂交DNA中该片段的存在与杂交细胞的人类染色体含量相关联，PTH基因被定位在11号染色体的短臂上。

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Human parathyroid hormone gene (PTH) is on short arm of chromosome 11.

The human gene for parathyroid hormone (PTH) was chromosomally mapped using human-rodent hybrids and Southern filter hybridization of cell hybrid DNA. A recombinant DNA probe containing human PTH cDNA insert (pPTHm122) hybridized to a 3.7-kb fragment in human DNA cleaved with the restriction enzyme EcoRI. By correlating the presence of this fragment in somatic cell hybrid DNA with the human chromosomal content of the hybrid cells, the PTH gene was mapped to the short arm of the chromosome 11.

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Somatic Cell Genetics

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