适度近交法开发脂肪糖酵母菌种源。

C Kurischko, S G Inge-Vechtomov, H Weber
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引用次数: 0

摘要

采用兄妹杂交的方法,改良了一株利用烷烃的脂肪糖酵母菌野生菌株的产孢参数。产孢频率、四孢子子囊数和子囊孢子活力均显著提高。迄今为止,许多遗传上定义明确的菌株具有良好的产孢参数,并显示出适合遗传分析的标记的1:1分离模式。
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Development of breeding stocks of the yeast Saccharomycopsis lipolytica by methods of moderate inbreeding.

Sporulation parameters of genetically labelled strains, derived from a wild strain of the alkane-utilizing yeast Saccharomycopsis lipolytica were improved by a breeding program using brother-sister crosses. Sporulation frequency, the number of four-spored asci and viability of ascospores could be significantly enhanced. To date a number of genetically well-defined strains is available that have good sporulation parameters and show a 1:1 segregation pattern of markers suitable for genetic analysis.

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