高效液相色谱法分离测定抗球虫硫胺素类似物。

Acta vitaminologica et enzymologica Pub Date : 1984-01-01

K Nosaka, H Nishimura, A Iwashima

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摘要

抗球虫硫胺素类似物氯乙基硫胺素和二甲基硫胺的分离测定，硫胺素经碱性氧化转化为相应的硫代色素后，采用反相高效液相色谱法实现。结果表明，氯乙基硫胺素在酿酒酵母的非增殖细胞中积累的机制与硫胺素和二甲基的转运机制相同。

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Separate determination of anticoccidial thiamine analogs by high-performance liquid chromatography.

The separate determination of anticoccidial thiamine analogs such as chloroethylthiamine and dimethialium, and thiamine was achieved by reversed-phase high-performance liquid chromatography after conversion to the corresponding thiochromes by alkaline oxidation. Using this method, it was demonstrated that chloroethylthiamine is accumulated in nonproliferating cells of Saccharomyces cerevisiae by the same transport mechanism as for thiamine and dimethialium.

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Acta vitaminologica et enzymologica

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