{"title":"[在合成培养基中产生热不稳定的大肠杆菌肠毒素及其在Vero细胞培养上的试验]。","authors":"Y Germani, B Dassy","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A modification of the technique described by Evans for the production of Escherichia coli heat-labile enterotoxin was employed. Instead of Casamino yeast extract medium, which is toxic for Vero cells, Eagle's minimal essential medium (MEM) commonly used in cell cultures was substituted and the LT toxin produced in MEM was assayed on Vero cells.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"135B 3","pages":"291-5"},"PeriodicalIF":0.0000,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Production of heat-labile Escherichia coli enterotoxin in a synthetic medium and its assay on a culture of Vero cells].\",\"authors\":\"Y Germani, B Dassy\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A modification of the technique described by Evans for the production of Escherichia coli heat-labile enterotoxin was employed. Instead of Casamino yeast extract medium, which is toxic for Vero cells, Eagle's minimal essential medium (MEM) commonly used in cell cultures was substituted and the LT toxin produced in MEM was assayed on Vero cells.</p>\",\"PeriodicalId\":7904,\"journal\":{\"name\":\"Annales de microbiologie\",\"volume\":\"135B 3\",\"pages\":\"291-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annales de microbiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annales de microbiologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
对埃文斯描述的生产大肠杆菌热不稳定肠毒素的技术进行了改进。代替对Vero细胞有毒的Casamino yeast extract培养基，代之以细胞培养中常用的Eagle的minimal essential medium (MEM)，并在Vero细胞上检测MEM中产生的LT毒素。
[Production of heat-labile Escherichia coli enterotoxin in a synthetic medium and its assay on a culture of Vero cells].
A modification of the technique described by Evans for the production of Escherichia coli heat-labile enterotoxin was employed. Instead of Casamino yeast extract medium, which is toxic for Vero cells, Eagle's minimal essential medium (MEM) commonly used in cell cultures was substituted and the LT toxin produced in MEM was assayed on Vero cells.
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