牛精细胞活力的流式细胞术分析。

L Mátyus, G Szabó, I Resli, R Gáspár, S Damjanovich
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引用次数: 0

摘要

采用双醋酸荧光素和碘化丙啶双荧光标记联合55℃处理15 min,用流式细胞术监测精子细胞的活力和耐热性。从已知生育值的公牛身上获得的精子样本表明,该详细测试在检测生育差异方面的适用性。根据初步测试,这种方法似乎也适用于测试其他雄性动物的生育能力。该方法简单、快速,可为雄性动物的精液用于大规模人工授精提供一种新的筛选方法。
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Flow cytometric analysis of viability of bull sperm cells.

Fluorescein diacetate and propidium iodide double fluorescence labeling of animal sperm cells in combination with 55 degrees C treatment for 15 min was used to monitor the viability and heat tolerance of sperm cells by flow cytometry. The applicability of the elaborated test to detect fertility differences has been shown on sperm samples obtained from bulls of known fertility values. According to preliminary examinations the method seems to be suitable to test the fertility of other male animals, too. The simplicity of the relatively quick procedure may provide a new screening method to qualify male animals before using their semen for large scale artificial insemination.

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