{"title":"大鼠血清中免疫复合物测定的蛋白质适应性结合试验。","authors":"W M Siag, J M Jones","doi":"10.3109/08820138309066870","DOIUrl":null,"url":null,"abstract":"<p><p>We describe a simple and sensitive method for the detection of circulating immune complexes (CIC) in rats and have applied the method to test sera from tumor-bearing rats. CIC were precipitated preferentially with 6% polyethylene glycol, dissolved, and then bound to Staphylococcus aureus that are Protein A (PA) positive. Rabbit-anti-rat IgG (RARG) antibodies were then added and followed by 125I-PA. The RARG sandwich antibody enhanced the sensitivity of detection of CIC in rats at least 5 fold compared to that observed using 125I-PA without RARG.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 2","pages":"215-21"},"PeriodicalIF":0.0000,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309066870","citationCount":"0","resultStr":"{\"title\":\"Adaptation of a protein a binding assay for measurement of immune complexes in sera of rats.\",\"authors\":\"W M Siag, J M Jones\",\"doi\":\"10.3109/08820138309066870\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We describe a simple and sensitive method for the detection of circulating immune complexes (CIC) in rats and have applied the method to test sera from tumor-bearing rats. CIC were precipitated preferentially with 6% polyethylene glycol, dissolved, and then bound to Staphylococcus aureus that are Protein A (PA) positive. Rabbit-anti-rat IgG (RARG) antibodies were then added and followed by 125I-PA. The RARG sandwich antibody enhanced the sensitivity of detection of CIC in rats at least 5 fold compared to that observed using 125I-PA without RARG.</p>\",\"PeriodicalId\":13417,\"journal\":{\"name\":\"Immunological communications\",\"volume\":\"12 2\",\"pages\":\"215-21\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/08820138309066870\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunological communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/08820138309066870\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138309066870","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Adaptation of a protein a binding assay for measurement of immune complexes in sera of rats.
We describe a simple and sensitive method for the detection of circulating immune complexes (CIC) in rats and have applied the method to test sera from tumor-bearing rats. CIC were precipitated preferentially with 6% polyethylene glycol, dissolved, and then bound to Staphylococcus aureus that are Protein A (PA) positive. Rabbit-anti-rat IgG (RARG) antibodies were then added and followed by 125I-PA. The RARG sandwich antibody enhanced the sensitivity of detection of CIC in rats at least 5 fold compared to that observed using 125I-PA without RARG.
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