{"title":"聚甲基丙烯酸缩水甘油酯微球及其衍生物作为固定化蛋白质载体的制备。","authors":"S Hosaka, Y Murao, S Masuko, K Miura","doi":"10.3109/08820138309051968","DOIUrl":null,"url":null,"abstract":"<p><p>Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5 micron. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized gamma-globulin on the microspheres was found to be about 6 mg/m2. It was shown that dyed microspheres with a diameter of 2-4 micron are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":"12 5","pages":"509-17"},"PeriodicalIF":0.0000,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051968","citationCount":"17","resultStr":"{\"title\":\"Preparation of microspheres of poly(glycidyl methacrylate) and its derivatives as carriers for immobilized proteins.\",\"authors\":\"S Hosaka, Y Murao, S Masuko, K Miura\",\"doi\":\"10.3109/08820138309051968\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5 micron. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized gamma-globulin on the microspheres was found to be about 6 mg/m2. It was shown that dyed microspheres with a diameter of 2-4 micron are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.</p>\",\"PeriodicalId\":13417,\"journal\":{\"name\":\"Immunological communications\",\"volume\":\"12 5\",\"pages\":\"509-17\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/08820138309051968\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunological communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/08820138309051968\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138309051968","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparation of microspheres of poly(glycidyl methacrylate) and its derivatives as carriers for immobilized proteins.
Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5 micron. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized gamma-globulin on the microspheres was found to be about 6 mg/m2. It was shown that dyed microspheres with a diameter of 2-4 micron are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.
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