{"title":"两种线粒体DNA在中国仓鼠-小鼠体细胞杂交体中的繁殖。","authors":"L DeFrancesco","doi":"10.1007/BF01544054","DOIUrl":null,"url":null,"abstract":"<p><p>Mouse-hamster hybrid cells were analyzed for the species of mitochondrial DNA (mtDNA) retained using Southern blotting and hybridization with highly labeled mitochondrial DNA probes. Initial analyses were performed as soon as there were 10(7) cells, which took between five and eight weeks from the time the fusion was performed (approximately 23 cell doublings). The majority of clones tested had detectable levels of both mouse and hamster mtDNA at first testing.</p>","PeriodicalId":21767,"journal":{"name":"Somatic Cell Genetics","volume":"9 1","pages":"133-9"},"PeriodicalIF":0.0000,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01544054","citationCount":"9","resultStr":"{\"title\":\"Propagation of two species of mitochondrial DNA in chinese hamster-mouse somatic cell hybrids.\",\"authors\":\"L DeFrancesco\",\"doi\":\"10.1007/BF01544054\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mouse-hamster hybrid cells were analyzed for the species of mitochondrial DNA (mtDNA) retained using Southern blotting and hybridization with highly labeled mitochondrial DNA probes. Initial analyses were performed as soon as there were 10(7) cells, which took between five and eight weeks from the time the fusion was performed (approximately 23 cell doublings). The majority of clones tested had detectable levels of both mouse and hamster mtDNA at first testing.</p>\",\"PeriodicalId\":21767,\"journal\":{\"name\":\"Somatic Cell Genetics\",\"volume\":\"9 1\",\"pages\":\"133-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/BF01544054\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Somatic Cell Genetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/BF01544054\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Somatic Cell Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF01544054","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Propagation of two species of mitochondrial DNA in chinese hamster-mouse somatic cell hybrids.
Mouse-hamster hybrid cells were analyzed for the species of mitochondrial DNA (mtDNA) retained using Southern blotting and hybridization with highly labeled mitochondrial DNA probes. Initial analyses were performed as soon as there were 10(7) cells, which took between five and eight weeks from the time the fusion was performed (approximately 23 cell doublings). The majority of clones tested had detectable levels of both mouse and hamster mtDNA at first testing.
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