Induction of mitochondrial phosphoenolpyruvate carboxykinase in the liver of Rana catesbeiana tadpole treated with 3,5,3′-triiodothyronine and undergoing natural metamorphosis

The rates of synthesis and degradation of mitochondrial phosphoenolpyruvate carboxykinase (GTP : oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32) in the liver of tadpoles in the two developmental stages (stages VIII and XXII), and in those treated with 3,5,3′-triiodothyronine, were studied by immunochemical techniques. The rate of synthesis of the enzyme was found to be accelerated at 9 h and 6 days after triiodothyronine administration and also during natural metamorphic climax. No difference was observed in the degradation rate of the enzyme between the tadpoles in the two stages, VIII and XXII.