{"title":"b型流感嗜血杆菌荚膜多糖的酶联免疫吸附试验(ELISA)检测和测定。","authors":"F W Tiller, E Diener","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A direct sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect Haemophilus influenzae type b capsular antigen. Using polystyrene as the solid phase and peroxidase-labelled rabbit antibody the assay detected the antigen in concentrations of 0.1 ng/ml. Linearity was achieved within the range of 1ng to 10 microgram/ml. Subtle measurements of Haemophilus influenzae type b capsular antigen in body fluids are possible through ELISA which is superior to counterimmunoelectrophoresis and latex-particle agglutination in this respect. ELISA should facilitate investigations concerning PRP pathogenic effects in experimental Hib infection as well as in human Hib disease.</p>","PeriodicalId":23929,"journal":{"name":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"248 4","pages":"488-93"},"PeriodicalIF":0.0000,"publicationDate":"1981-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Haemophilus influenzae type b capsular polysaccharide detection and measurement by an enzyme-linked immunosorbent assay (ELISA).\",\"authors\":\"F W Tiller, E Diener\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A direct sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect Haemophilus influenzae type b capsular antigen. Using polystyrene as the solid phase and peroxidase-labelled rabbit antibody the assay detected the antigen in concentrations of 0.1 ng/ml. Linearity was achieved within the range of 1ng to 10 microgram/ml. Subtle measurements of Haemophilus influenzae type b capsular antigen in body fluids are possible through ELISA which is superior to counterimmunoelectrophoresis and latex-particle agglutination in this respect. ELISA should facilitate investigations concerning PRP pathogenic effects in experimental Hib infection as well as in human Hib disease.</p>\",\"PeriodicalId\":23929,\"journal\":{\"name\":\"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"volume\":\"248 4\",\"pages\":\"488-93\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Haemophilus influenzae type b capsular polysaccharide detection and measurement by an enzyme-linked immunosorbent assay (ELISA).
A direct sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect Haemophilus influenzae type b capsular antigen. Using polystyrene as the solid phase and peroxidase-labelled rabbit antibody the assay detected the antigen in concentrations of 0.1 ng/ml. Linearity was achieved within the range of 1ng to 10 microgram/ml. Subtle measurements of Haemophilus influenzae type b capsular antigen in body fluids are possible through ELISA which is superior to counterimmunoelectrophoresis and latex-particle agglutination in this respect. ELISA should facilitate investigations concerning PRP pathogenic effects in experimental Hib infection as well as in human Hib disease.
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