小肠结肠炎耶尔森菌对人类补体的激活:超微结构改变和c3b沉积。

G Acker, V Brade
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引用次数: 0

摘要

在血清或不含溶菌酶的血清中观察到平滑形态的小肠结肠炎耶尔森菌75 (Ye 75s)的快速杀伤,而egta -血清的杀伤活性较慢,在加热(30 min 56℃)血清中无杀伤活性。同样,Ye 75 S在血清和无溶菌酶血清中激活补体(C)的速度很快,但在另一途径(egta -血清)中激活速度很慢。这些数据表明,C足以杀死细胞,并通过完整的经典途径最活跃。对血清(C +溶菌酶)或无溶菌酶血清(C)杀死的细菌进行了电镜观察。在这些实验中,Ye 75s暴露于血清后,细胞碎裂和球质体形成;在无溶菌酶血清培养的细菌中,最显著的变化是“水泡”的形成。这些水泡很可能起源于外膜,是细胞表面C活化的结果。在血清或egta血清存在的情况下,对活化C (C3b)在Ye 75s上的沉积进行动力学分析。血清(30体积%)在20- 30分钟内观察到大量C3b沉积,而egta血清(30体积%)C3b沉积较慢且不完全。egta血清实验也显示C3b的沉积开始于单个位点,主要位于细胞极区;C3b从这些部位开始扩散,直到细胞表面的大片区域被覆盖。这些数据表明,通过替代途径激活C仅限于细菌表面的某些区域。
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Activation of human complement by Yersinia enterocolitica: ultrastructural alterations and C3b-deposition.

Rapid killing of Yersinia enterocolitica strain 75 in smooth form (Ye 75 S) was observed in the presence of serum or of lysozyme-free serum whereas the killing activity of EGTA-serum was slow, and absent in heated (30 min 56 degree C) serum. Similarly, complement (C) activation by Ye 75 S was rapid in serum and lysozyme-free serum but slow via the alternative pathway (EGTA-serum). These data suggest that C is sufficient for killing of the cells and most active via an intact classical pathway. Electronmicroscopic studies were performed on bacterial killed by serum (C + lysozyme) or by lysozyme-free serum (C). In these experiments cell fragmentation and spheroplast formation were seen after exposure of Ye 75 S to serum; in bacteria incubated with lysozyme-free serum "blebs" formation was observed as the most prominent alteration. These blebs most likely originate from the outer membrane as a result of C activation on the cell surface. The deposition of activated C (C3b) on Ye 75 S was analyzed kinetically in the presence of serum or EGTA-serum. With serum (30 vol%) massive C3b deposition was observed within 20--30 min whereas with EGTA-serum (30 vol%) the deposition of C3b was slower and less complete. Experiments with EGTA-serum also revealed that the deposition of C3b started at single sites mainly located in the region of the cell poles; from these sites spreading of C3b occurred until large areas of the cell surface were covered. These data suggest that C activation via the alternative pathway is restricted to certain regions of the bacterial surface.

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[On the biological action of transition metal complexes. 1. The antiviral activity of palladium aminopyridin-complexes (author's transl)]. Increased serum antibacterial activity after turpentine-induced acute inflammation. Comparison of the effects of a multi-component vaccine and a formalin-killed cell vaccine on protection against enzootic of hemorrhagic pneumonia due to Pseudomonas aeruginosa in mink. Ultrastructural study of interaction of group A streptococci with tissue culture cells. [Results from the Central Laboratory for Streptococci Research in Kiel from 1965 to 1978 - Mastitis streptococci (author's transl)].
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