{"title":"体外造血祖细胞扩增","authors":"Haylock D.N., Makino S., Dowse T.L., Trimboli S., Niutta S., To L.B., Juttner C.A., Simmons P.J.","doi":"10.1006/immu.1994.1059","DOIUrl":null,"url":null,"abstract":"<div><p>The ability to culture and expand hematopoietic progenitor cells <em>ex vivo</em> has major implications for both bone marrow and stem cell support following marrow ablative or subablative high-dose therapy and for improving the efficiency of retroviral transfection in gene marking and gene therapy. This review focuses on methods for the generation of myeloid progenitor and post-progenitor cells from peripheral blood stem cell collections, with particular emphasis on the characterization of these cells and practical issues associated with their expansion.</p></div>","PeriodicalId":79341,"journal":{"name":"ImmunoMethods","volume":"5 3","pages":"Pages 217-225"},"PeriodicalIF":0.0000,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/immu.1994.1059","citationCount":"14","resultStr":"{\"title\":\"Ex Vivo Hematopoietic Progenitor Cell Expansion\",\"authors\":\"Haylock D.N., Makino S., Dowse T.L., Trimboli S., Niutta S., To L.B., Juttner C.A., Simmons P.J.\",\"doi\":\"10.1006/immu.1994.1059\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The ability to culture and expand hematopoietic progenitor cells <em>ex vivo</em> has major implications for both bone marrow and stem cell support following marrow ablative or subablative high-dose therapy and for improving the efficiency of retroviral transfection in gene marking and gene therapy. This review focuses on methods for the generation of myeloid progenitor and post-progenitor cells from peripheral blood stem cell collections, with particular emphasis on the characterization of these cells and practical issues associated with their expansion.</p></div>\",\"PeriodicalId\":79341,\"journal\":{\"name\":\"ImmunoMethods\",\"volume\":\"5 3\",\"pages\":\"Pages 217-225\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1006/immu.1994.1059\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ImmunoMethods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S105866878471059X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ImmunoMethods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S105866878471059X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The ability to culture and expand hematopoietic progenitor cells ex vivo has major implications for both bone marrow and stem cell support following marrow ablative or subablative high-dose therapy and for improving the efficiency of retroviral transfection in gene marking and gene therapy. This review focuses on methods for the generation of myeloid progenitor and post-progenitor cells from peripheral blood stem cell collections, with particular emphasis on the characterization of these cells and practical issues associated with their expansion.
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