[产科学用GBS培养基筛查B型链球菌]。

Immunitat und Infektion Pub Date : 1995-08-01
D Milatovic, C Wallrauch, A Voss, M Kolben, I Braveny
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引用次数: 0

摘要

对孕妇和新生儿进行B组链球菌(GBS)定植筛查,前者从子宫颈和尿道采集成对拭子,后者从耳、鼻、脐带、胃液和膜采集成对拭子。一个拭子在血琼脂上培养;另一株接种于血清淀粉肉汤(GBS培养基)中,通过产生一种特有的橙色色素来鉴定GBS。从获得的2105对拭子中,两种方法均有158例GBS阳性;其中,GBS培养基回收率为154(97.5%),血琼脂平板回收率为89(56.3%)。GBS培养基未见假阳性显色反应。75%的GBS阳性培养基可在孵育24小时内读取。使用GBS培养基被证明是一种简单可靠的筛选母体和新生儿GBS定植的方法。
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[Streptococcus B screening with GBS medium in obstetrics].

Pregnant women and newborn infants were screened for group B streptococcal (GBS) colonization by obtaining paired swabs from the cervix and urethra for the former group and from the ear, nose, umbilical cord, gastric juice and membranes for the latter. One swab was cultured on blood agar; the other was inoculated into serum-starch broth (GBS medium), which allows identification of GBS by production of a characteristic orange-colored pigment. From the 2105 paired swabs obtained, a total of 158 were GBS positive by either method; of these, 154 (97.5%) were recovered by the GBS medium and 89 (56.3%) by blood agar plate. No false positive color reactions were observed with GBS medium. 75% of the positive GBS media could be read within 24 h of incubation. The use of GBS medium proved to be an easy and reliable method for screening of maternal and neonatal GBS colonization.

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