非脂质利用菌对醋酸代谢的影响

Laura Kawasaki, Amelia Farrés, Jesús Aguirre
{"title":"非脂质利用菌对醋酸代谢的影响","authors":"Laura Kawasaki,&nbsp;Amelia Farrés,&nbsp;Jesús Aguirre","doi":"10.1006/emyc.1995.1009","DOIUrl":null,"url":null,"abstract":"<div><p>Kawasaki, L., Farrés, A., and Aguirre, J. 1995. <em>Aspergillus nidulans</em> mutants affected in acetate metabolism isolated as lipid nonutilizers. <em>Experimental Mycology</em> 19, 81-85. Interest in extracellular fungal lipases has increased mainly because of their industrial applications. However, no studies have been done on a genetically well characterized filamentous fungus like <em>Aspergillus nidulans</em>. Here we show that <em>A. nidulans</em> produces an extracellular lipase when grown in solid or liquid cultures containing lipids as carbon source. This lipase is glucose-repressed in a <em>creA</em>-independent fashion. Seven mutants isolated by their inability to utilize lipids as sole carbon source were also unable to utilize acetate as sole carbon source. Representative mutants from each of three complementation groups were tested for allelism with strains carrying well known mutations affecting acetate metabolism. They were found to contain <em>acuD</em> (Isocitrate lyase), <em>acuF</em> (PEP carboxykinase), and <em>acuE</em> (Malate synthase) alleles. Screening of lipid nonutilizing mutants for growth in acetate provides a method for the isolation of both lipase minus and new acetate metabolism mutants.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"19 1","pages":"Pages 81-85"},"PeriodicalIF":0.0000,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1995.1009","citationCount":"17","resultStr":"{\"title\":\"Aspergillus nidulans Mutants Affected in Acetate Metabolism Isolated as Lipid Nonutilizers\",\"authors\":\"Laura Kawasaki,&nbsp;Amelia Farrés,&nbsp;Jesús Aguirre\",\"doi\":\"10.1006/emyc.1995.1009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Kawasaki, L., Farrés, A., and Aguirre, J. 1995. <em>Aspergillus nidulans</em> mutants affected in acetate metabolism isolated as lipid nonutilizers. <em>Experimental Mycology</em> 19, 81-85. Interest in extracellular fungal lipases has increased mainly because of their industrial applications. However, no studies have been done on a genetically well characterized filamentous fungus like <em>Aspergillus nidulans</em>. Here we show that <em>A. nidulans</em> produces an extracellular lipase when grown in solid or liquid cultures containing lipids as carbon source. This lipase is glucose-repressed in a <em>creA</em>-independent fashion. Seven mutants isolated by their inability to utilize lipids as sole carbon source were also unable to utilize acetate as sole carbon source. Representative mutants from each of three complementation groups were tested for allelism with strains carrying well known mutations affecting acetate metabolism. They were found to contain <em>acuD</em> (Isocitrate lyase), <em>acuF</em> (PEP carboxykinase), and <em>acuE</em> (Malate synthase) alleles. Screening of lipid nonutilizing mutants for growth in acetate provides a method for the isolation of both lipase minus and new acetate metabolism mutants.</p></div>\",\"PeriodicalId\":12110,\"journal\":{\"name\":\"Experimental Mycology\",\"volume\":\"19 1\",\"pages\":\"Pages 81-85\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1006/emyc.1995.1009\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147597585710092\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597585710092","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17

摘要

Kawasaki, L., farrsamas, A.和Aguirre, J. 1995。中性曲霉突变体对脂质代谢的影响。实验真菌学,19,81-85。对细胞外真菌脂肪酶的兴趣增加主要是因为它们的工业应用。然而,目前还没有对一种具有良好遗传特征的丝状真菌进行研究,比如细粒曲霉。在这里,我们表明,当在含有脂质作为碳源的固体或液体培养物中生长时,芽孢杆菌产生细胞外脂肪酶。这种脂肪酶以一种不依赖于氨基酸的方式抑制葡萄糖。7个不能利用脂质作为唯一碳源的突变体也不能利用醋酸作为唯一碳源。来自三个互补组的代表性突变体与携带影响乙酸代谢的已知突变的菌株进行等位基因检测。发现它们含有acuD(异柠檬酸裂解酶),acuF (PEP羧激酶)和acuE(苹果酸合成酶)等位基因。筛选脂质不利用突变体在乙酸中生长,为分离脂肪酶缺失突变体和新的醋酸代谢突变体提供了一种方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Aspergillus nidulans Mutants Affected in Acetate Metabolism Isolated as Lipid Nonutilizers

Kawasaki, L., Farrés, A., and Aguirre, J. 1995. Aspergillus nidulans mutants affected in acetate metabolism isolated as lipid nonutilizers. Experimental Mycology 19, 81-85. Interest in extracellular fungal lipases has increased mainly because of their industrial applications. However, no studies have been done on a genetically well characterized filamentous fungus like Aspergillus nidulans. Here we show that A. nidulans produces an extracellular lipase when grown in solid or liquid cultures containing lipids as carbon source. This lipase is glucose-repressed in a creA-independent fashion. Seven mutants isolated by their inability to utilize lipids as sole carbon source were also unable to utilize acetate as sole carbon source. Representative mutants from each of three complementation groups were tested for allelism with strains carrying well known mutations affecting acetate metabolism. They were found to contain acuD (Isocitrate lyase), acuF (PEP carboxykinase), and acuE (Malate synthase) alleles. Screening of lipid nonutilizing mutants for growth in acetate provides a method for the isolation of both lipase minus and new acetate metabolism mutants.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Biosynthetic Pathways of Glycerol Accumulation under Salt Stress in Aspergillus nidulans Involvement of Candida albicans Cell Wall Proteins in the Adherence of Blastospores to Human Buccal Epithelial Cells Extraction and Characterization of the Insecticidal Toxin Hirsutellin A Produced by Hirsutella thompsonii var. thompsonii Superoxide Dismutase: A Differentiation Protein Expressed in Uromyces Germlings during Early Appressorium Development A Unique Repeated DNA Sequence in the Cyclosporin-Producing Strain of Tolypocladium inflatum (ATCC 34921)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1