{"title":"肺泡巨噬细胞微核测定，一种检测环境物质经吸入途径进入的遗传毒性的简单模型","authors":"K. Sahu, R.K. Das","doi":"10.1016/0165-7992(95)90071-3","DOIUrl":null,"url":null,"abstract":"<div><p>A simple and short-term micronucleus (MN) test in pulmonary alveolar macrophages (PAMs) of rats has been developed to assess potential genotoxic effects of gaseous environmental agents. The protocol has been tested in model experiments with indoor air pollutants like mosquito coil smoke (MCS) and mosquito mat vapour (MMV). Smears of pulmonary lavage fluid collected in hypotonic (0.56%) KC1 solution were fixed in absolute methanol and stained in Giemsa (10%). Characteristically the large size of the PAMs facilitates easy scoring of MN. An interval of 32 h post exposure seems to be suitable for MN preparation. A comparison of the concentration-response data on CAs (at 24 h post exposure) and MN (at 32 h post exposure) clearly reveals the validity of the MN assay in PAMs</p></div>","PeriodicalId":100934,"journal":{"name":"Mutation Research Letters","volume":"347 2","pages":"Pages 61-65"},"PeriodicalIF":0.0000,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0165-7992(95)90071-3","citationCount":"3","resultStr":"{\"title\":\"Micronucleus assay in pulmonary alveolar macrophages, a simple model to detect genotoxicity of environmental agents entering through the inhalation route\",\"authors\":\"K. Sahu, R.K. Das\",\"doi\":\"10.1016/0165-7992(95)90071-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A simple and short-term micronucleus (MN) test in pulmonary alveolar macrophages (PAMs) of rats has been developed to assess potential genotoxic effects of gaseous environmental agents. The protocol has been tested in model experiments with indoor air pollutants like mosquito coil smoke (MCS) and mosquito mat vapour (MMV). Smears of pulmonary lavage fluid collected in hypotonic (0.56%) KC1 solution were fixed in absolute methanol and stained in Giemsa (10%). Characteristically the large size of the PAMs facilitates easy scoring of MN. An interval of 32 h post exposure seems to be suitable for MN preparation. A comparison of the concentration-response data on CAs (at 24 h post exposure) and MN (at 32 h post exposure) clearly reveals the validity of the MN assay in PAMs</p></div>\",\"PeriodicalId\":100934,\"journal\":{\"name\":\"Mutation Research Letters\",\"volume\":\"347 2\",\"pages\":\"Pages 61-65\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0165-7992(95)90071-3\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0165799295900713\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research Letters","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0165799295900713","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Micronucleus assay in pulmonary alveolar macrophages, a simple model to detect genotoxicity of environmental agents entering through the inhalation route
A simple and short-term micronucleus (MN) test in pulmonary alveolar macrophages (PAMs) of rats has been developed to assess potential genotoxic effects of gaseous environmental agents. The protocol has been tested in model experiments with indoor air pollutants like mosquito coil smoke (MCS) and mosquito mat vapour (MMV). Smears of pulmonary lavage fluid collected in hypotonic (0.56%) KC1 solution were fixed in absolute methanol and stained in Giemsa (10%). Characteristically the large size of the PAMs facilitates easy scoring of MN. An interval of 32 h post exposure seems to be suitable for MN preparation. A comparison of the concentration-response data on CAs (at 24 h post exposure) and MN (at 32 h post exposure) clearly reveals the validity of the MN assay in PAMs
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