Dandan Xu , Xiaoyan Ju , Meng Zhu , Jinzhao Ou , Guojun Lu , Chenxiao Wan , Kejia Li , Wei Jiang , Xinyu Jia , Yongping Han , Ye Tian , Zhongwei Niu
{"title":"亮氨酸四肽表面修饰:一种抗革兰氏阴性菌的抗菌策略","authors":"Dandan Xu , Xiaoyan Ju , Meng Zhu , Jinzhao Ou , Guojun Lu , Chenxiao Wan , Kejia Li , Wei Jiang , Xinyu Jia , Yongping Han , Ye Tian , Zhongwei Niu","doi":"10.1016/j.jcis.2023.03.038","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span><span>Surface-associated microbe contamination by Gram-negative bacteria poses a serious problem in medical care. Cationic peptides or polymers are the main materials used for antibacterial surface coating, but the positive charge may lead to </span>blood coagulation. Therefore, exploiting surface coating which is free of positive charge and is effective for Gram-negative bacteria inactivation is in urgent need. In this study, inspired by the affinity between </span>lipopolysaccharides of Gram-negative bacteria and Toll-like receptors of immune cells, we develop a leucine-based </span>tetrapeptide coating strategy for combating Gram-negative bacteria. The obtained surface has excellent bactericidal activity against Gram-negative bacteria like </span><span><em>Pseudomonas</em><em> aeruginosa</em></span> and <em>Escherichia coli</em>. A 1 mm<sup>2</sup> coated glass surface could kill > 9.9 × 10<sup>4</sup><span> CFU bacteria in 1 h and has nearly no damage to mammal cells. Moreover, this surface coating strategy could be applied on various surfaces like glass slices, glass capillary cavity and thermoplastic<span> polyurethane slices. And the coated surface could largely mitigate the microbe contamination in an </span></span><em>in vivo</em> subcutaneous implantation. This work paves a new way for antibacterial surface-coating which is behaving no positive charge and is of great importance for biomedical devices.</p></div>","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":null,"pages":null},"PeriodicalIF":9.4000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Surface decoration with leucine tetrapeptide: An antibacterial strategy against Gram-negative bacteria\",\"authors\":\"Dandan Xu , Xiaoyan Ju , Meng Zhu , Jinzhao Ou , Guojun Lu , Chenxiao Wan , Kejia Li , Wei Jiang , Xinyu Jia , Yongping Han , Ye Tian , Zhongwei Niu\",\"doi\":\"10.1016/j.jcis.2023.03.038\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span><span><span>Surface-associated microbe contamination by Gram-negative bacteria poses a serious problem in medical care. Cationic peptides or polymers are the main materials used for antibacterial surface coating, but the positive charge may lead to </span>blood coagulation. Therefore, exploiting surface coating which is free of positive charge and is effective for Gram-negative bacteria inactivation is in urgent need. In this study, inspired by the affinity between </span>lipopolysaccharides of Gram-negative bacteria and Toll-like receptors of immune cells, we develop a leucine-based </span>tetrapeptide coating strategy for combating Gram-negative bacteria. The obtained surface has excellent bactericidal activity against Gram-negative bacteria like </span><span><em>Pseudomonas</em><em> aeruginosa</em></span> and <em>Escherichia coli</em>. A 1 mm<sup>2</sup> coated glass surface could kill > 9.9 × 10<sup>4</sup><span> CFU bacteria in 1 h and has nearly no damage to mammal cells. Moreover, this surface coating strategy could be applied on various surfaces like glass slices, glass capillary cavity and thermoplastic<span> polyurethane slices. And the coated surface could largely mitigate the microbe contamination in an </span></span><em>in vivo</em> subcutaneous implantation. This work paves a new way for antibacterial surface-coating which is behaving no positive charge and is of great importance for biomedical devices.</p></div>\",\"PeriodicalId\":351,\"journal\":{\"name\":\"Journal of Colloid and Interface Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.4000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Colloid and Interface Science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0021979723003971\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Colloid and Interface Science","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021979723003971","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Surface decoration with leucine tetrapeptide: An antibacterial strategy against Gram-negative bacteria
Surface-associated microbe contamination by Gram-negative bacteria poses a serious problem in medical care. Cationic peptides or polymers are the main materials used for antibacterial surface coating, but the positive charge may lead to blood coagulation. Therefore, exploiting surface coating which is free of positive charge and is effective for Gram-negative bacteria inactivation is in urgent need. In this study, inspired by the affinity between lipopolysaccharides of Gram-negative bacteria and Toll-like receptors of immune cells, we develop a leucine-based tetrapeptide coating strategy for combating Gram-negative bacteria. The obtained surface has excellent bactericidal activity against Gram-negative bacteria like Pseudomonas aeruginosa and Escherichia coli. A 1 mm2 coated glass surface could kill > 9.9 × 104 CFU bacteria in 1 h and has nearly no damage to mammal cells. Moreover, this surface coating strategy could be applied on various surfaces like glass slices, glass capillary cavity and thermoplastic polyurethane slices. And the coated surface could largely mitigate the microbe contamination in an in vivo subcutaneous implantation. This work paves a new way for antibacterial surface-coating which is behaving no positive charge and is of great importance for biomedical devices.
期刊介绍:
The Journal of Colloid and Interface Science publishes original research findings on the fundamental principles of colloid and interface science, as well as innovative applications in various fields. The criteria for publication include impact, quality, novelty, and originality.
Emphasis:
The journal emphasizes fundamental scientific innovation within the following categories:
A.Colloidal Materials and Nanomaterials
B.Soft Colloidal and Self-Assembly Systems
C.Adsorption, Catalysis, and Electrochemistry
D.Interfacial Processes, Capillarity, and Wetting
E.Biomaterials and Nanomedicine
F.Energy Conversion and Storage, and Environmental Technologies