[口服百日咳博德氏杆菌表面抗原免疫后肺淋巴细胞产生抗体增强]。

Immunitat und Infektion Pub Date : 1994-06-01

M Frühwirth, H Ellemunter, C Ruedl, H Wolf

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引用次数: 0

摘要

本研究的目的是评价口服百日咳表面抗原疫苗接种对抗原施用部位免疫应答的影响。我们连续5天口服免疫雌性BALB/c小鼠，每隔10天自由间隔重复这一步骤。分离肺淋巴细胞(LL)、Peyer’s patches (PPL)和肠固有层淋巴细胞(LPL)，用时间分辨免疫荧光法测定免疫球蛋白分泌率。口服免疫可使LL的IgA分泌率比未免疫动物提高69.9%。血清中IgG和IgM的合成分别提高28.1%和14.1%。此外，LPL和PPL的IgG分泌量提高了47.8%。因此，我们的研究结果表明，口服百日咳博德泰拉免疫后，局部免疫反应很强。

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[Enhanced antibody production by lung lymphocytes after oral immunization with Bordetella pertussis surface antigens].

The purpose of our study was to evaluate the effect of oral vaccination with Bordetella pertussis surface antigens on the immune response at the site of antigen application. We orally immunized female BALB/c mice on five consecutive days and repeated this procedure after a free interval of 10 days. Lymphocytes of the lung (LL), Peyer's patches (PPL) and lamina propria of the gut (LPL) were isolated and the immunoglobulin secretion rate was measured with time-resolved immunofluorescence. Oral immunization was found to enhance the IgA secretion rate by 69.9% in LL compared to unimmunized animals. The IgG synthesis in LL was increased by 28.1% and the IgM synthesis by 14.1%. In addition, an improvement of 47.8% was observed for the IgG secretion in LPL and PPL. Thus, our results demonstrate a strong local immune response after oral immunization with Bordetella pertussis.

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Immunitat und Infektion

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