早产儿凝固酶阴性葡萄球菌表面菌群的分子特征。

H Bialkowska-Hobrzanska, D Jaskot, O Hammerberg
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引用次数: 18

摘要

进行了一项单点研究,以确定哪些表面位点最能代表早产儿凝固酶阴性葡萄球菌(CNS)定殖菌群的密度和组成。随机选择6名在新生儿重症监护病房(NICU)住院1个月的新生儿,对其5个不同的体表部位进行了检查。利用全染色体DNA和核糖体RNA基因的限制性内切酶指纹图谱对CNS种内的菌株及其克隆组织进行鉴定。收集头皮、脐部、足部、鼻子和直肠的培养物并进行定量处理。每个表面培养分型10个菌落。NICU新生儿的CNS定植密度最高的部位为脐部(平均1.2 × 10(4) c.f.u. cm-2)、足部(平均1.6 × 10(3) c.f.u. cm-2)和鼻子(平均1.7 × 10(3) c.f.u. cm-2)。头皮和直肠几乎没有定植。CNS表面分离株中表皮葡萄球菌占77.7%(219/282),溶血葡萄球菌占20.6%(58/282),瓦尔纳氏葡萄球菌占1.4%(4/282),头孢葡萄球菌占0.4%(1/282)。每个表面位点的定植最多包括5个不同的菌株,代表4个中枢神经系统物种。282株分离物中检出5株表皮葡萄球菌、2株溶血葡萄球菌、1株沃纳氏葡萄球菌和1株猪链球菌。其中表皮葡萄球菌2个无性系和溶血葡萄球菌1个无性系最为优势;他们占94%(265/282)。来自足部和头皮的培养物代表了五个研究地点中最异质的中枢神经系统定植。(摘要删节250字)
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Molecular characterization of the coagulase-negative staphylococcal surface flora of premature neonates.

A single point study was conducted to determine which surface sites best represent the density and composition of the coagulase-negative staphylococcal (CNS) colonizing flora in premature neonates. Five different surface sites of six randomly selected neonates hospitalized in a neonatal intensive care unit (NICU) for a month were examined. The individual strains and their clonal organization within CNS species were identified using restriction endonuclease fingerprinting of whole chromosomal DNA and ribosomal RNA genes. Cultures of the scalp, umbilicus, foot, nose and rectum were collected and quantitatively processed. Ten colonies were typed per surface culture. The most dense CNS colonization was noted on the umbilicus (mean 1.2 x 10(4) c.f.u. cm-2), foot (mean 1.6 x 10(3) c.f.u. cm-2) and nose (mean 1.7 x 10(3) c.f.u. cm-2) of NICU neonates. Scalp and rectum were scarcely colonized. Of all the CNS surface isolates, S. epidermidis accounted for 77.7% (219/282) and S. haemolyticus, S. warneri and S. capitis accounted for 20.6% (58/282), 1.4% (4/282) and 0.4% (1/282), respectively. Colonization of each surface site comprised a maximum of five different strains representing four CNS species. Overall, five clones of S. epidermidis, two of S. haemolyticus, one of S. warneri and one of S. capitis were noted among the 282 isolates. The most predominant were two clones of S. epidermidis and one of S. haemolyticus; they accounted for 94% (265/282). Cultures from the foot and scalp represented the most heterogeneous CNS colonization of the five sites examined.(ABSTRACT TRUNCATED AT 250 WORDS)

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