猪皮肤葡萄球菌的四环素耐药基因。

S Schwarz, W C Noble
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引用次数: 51

摘要

对来自猪皮肤的47株四环素耐药葡萄球菌进行了检测,以寻找介导这种耐药的基因。17株菌株对二甲胺四环素也有耐药性,且均与tet(M)基因杂交;23株携带tet(K)基因,10株携带tet(L)基因。其中三个携带不止一个基因,两个没有与三种探针中的任何一种杂交。两个携带tet(K)基因的质粒在大小(4.35 ~ 4.7 kb)和结构上都非常相似,与质粒pT181非常相似。携带tet(L)基因的4个质粒大小不同,大小范围在4.3 ~ 11.5 kb之间,除了携带该基因的部分外,其余质粒结构也不相同。
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Tetracycline resistance genes in staphylococci from the skin of pigs.

Forty-seven tetracycline-resistant staphylococci from the skin of pigs were examined for genes mediating this resistance. Seventeen isolates were also resistant to minocycline and all hybridized with the tet(M) gene; 23 carried the tet(K) gene and 10 the tet(L) gene. Three carried more than one gene and two did not hybridize with any of the three probes tested. Maps were constructed for two plasmids carrying the tet(K) gene, all were very similar in size (4.35-4.7 kb) and structure and closely resembled the plasmid pT181. Four plasmids which bore the tet(L) gene differed in size, ranging from 4.3 to 11.5 kb, and were dissimilar in structure except for the portion bearing the gene.

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