琼脂糖凝胶多位点酶电泳技术在昆虫致病性球形芽孢杆菌菌株鉴定中的应用。

V Zahner, L Rabinovitch, C F Cavados, H Momen
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引用次数: 20

摘要

对60株球形芽孢杆菌(Bacillus sphaericus)进行了多位点酶电泳研究,将其分为44个酶型(电泳型)。昆虫致病菌株中有11株属于同一菌株(Z59),表明该菌株具有广泛的频繁基因型。GPI (E.C.5.3.1.9)、G6P (E.C.1.1.1.49)、6PG (E.C.1.1.1.44)和ME (E.C.1.1.1.40)位点未检测到酶活性条带。酶位点NP (E.C.2.4.2.1)和ACON (E.C.4.2.1.3)为单态,其他酶位点MDH (E.C.1.1.1.37)、LeDH (E.C.1.4.1.9)、ADH (E.C.1.4.1.1)、EST (E.C.3.1.1.1)、PEP-2 (E.C.3.4.11.1)、PEP-3 (E.C.3.4.11)和PEP-D (E.C. 3.4.13.9)为多态性。非昆虫致病组的遗传变异似乎大于昆虫致病组。后一组似乎不同于这些物种的其他菌株。所有昆虫病原菌均在同一表型群中恢复,并报道了鉴定昆虫病原菌株的诊断等位基因。
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Multilocus enzyme electrophoresis on agarose gel as an aid to the identification of entomopathogenic Bacillus sphaericus strains.

Sixty strains of Bacillus sphaericus, including 31 insect pathogens were studied by multilocus enzyme electrophoresis and were classified into 44 zymovars (electrophoretic types). Among the entomopathogenic strains, 11 belong to the same zymovar (Z59) indicating a widespread frequent genotype. Bands of enzyme activity were not detected among the strains for the loci GPI (E.C.5.3.1.9), G6P (E.C.1.1.1.49), 6PG (E.C.1.1.1.44) and ME (E.C.1.1.1.40). The enzymatic loci NP (E.C.2.4.2.1) and ACON (E.C.4.2.1.3) were monomorphic while the other enzymes, MDH (E.C.1.1.1.37), LeDH (E.C.1.4.1.9), ADH (E.C.1.4.1.1), EST (E.C.3.1.1.1), PEP-2 (E.C.3.4.11.1), PEP-3 (E.C.3.4.11) and PEP-D (E.C. 3.4.13.9) were polymorphic. The genetic variation in the non-insect pathogenic group seemed to be greater than in the entomopathogenic group. This latter group appears to be distinct from other strains of these species. All insect pathogens were recovered in the same phenetic cluster and a diagnostic allele is reported for the identification of entomopathogenic strains.

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