荧光假单胞菌部分纯化蛋白酶P-26的耐热性。

V K Uplacksh, D K Mathur, R K Malik
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引用次数: 4

摘要

采用(NH4)2 SO4沉淀(饱和度45-65%)和1.0 ~ 2.0体积异丙醇溶剂分馏的方法对荧光假单胞菌P-26蛋白酶进行部分纯化,在72.5、130、135、140、145和150℃条件下,对全脂牛奶、脱脂牛奶、乳清和0.05 mol l-1磷酸缓冲液中P-26蛋白酶的耐热性d值进行了研究。在全脂牛奶中测定的蛋白酶在所有温度下的d值最大(D150 = 0.088)。
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Thermal resistance of partially purified proteinase of Pseudomonas fluorescens P-26.

Heat resistance of the Pseudomonas fluorescens P-26 proteinase in terms of D-value was studied in whole milk, skim milk, whey and 0.05 mol l-1 phosphate buffer at 72.5, 130, 135, 140, 145 and 150 degrees C subsequent to its partial purification through (NH4)2 SO4 precipitation (45-65% saturation) and solvent fractionation with 1.0 to 2.0 volumes of isopropanol. The D-value was maximum for the proteinase at all temperatures when determined in whole milk (D150 = 0.088).

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