底物对枯草芽孢杆菌抗真菌挥发物产生的影响。

P J Fiddaman, S Rossall
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引用次数: 132

摘要

一种产生抗生素的枯草芽孢杆菌菌株已被证明能产生有效的抗真菌挥发物(AFV)。这些挥发物对一系列真菌物种有活性,并在一系列生长介质和壤土堆肥中产生。在琼脂上添加d -葡萄糖、复合碳水化合物和蛋白胨增强了体外抗真菌挥发活性。与同等水平的d -葡萄糖相比,添加l -葡萄糖导致AFV活性显著降低。液体培养的生长研究表明,枯草芽孢杆菌在l -葡萄糖的作用下不能生长。在固体培养基上的进一步生长研究表明,细菌生长的增强与体外AFV活性的增加之间没有明显的相关性,这是对基质供应的反应。在接种枯草芽孢杆菌的油菜根部也检测到低水平的AFV活性。气相色谱-质谱顶空分析显示,在不同底物上生长的枯草芽孢杆菌培养物在促进AFV活性的底物之间存在共同的相似性,尽管不可能分离出单个抗真菌化合物。
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Effect of substrate on the production of antifungal volatiles from Bacillus subtilis.

An antibiotic-producing strain of Bacillus subtilis has been shown to produce potent antifungal volatiles (AFV). These volatiles are active against a range of fungal species and are produced on a range of growth media and in loam-based compost. In vitro antifungal volatile activity on nutrient agar is enhanced with the addition of D-glucose, complex carbohydrates and peptones. The addition of L-glucose led to significantly less AFV activity than comparable levels of D-glucose. Growth studies in liquid culture revealed that B. subtilis failed to grow in response to L-glucose. Further growth studies on solid media showed no clear correlation between enhanced bacterial growth and increases in in vitro AFV activity in response to supply of substrates. Low level AFV activity was also detected from oilseed rape roots inoculated with B. subtilis. Gas chromatography mass spectrometry headspace analysis of B. subtilis cultures grown on various substrates revealed common similarities between substrates promoting AFV activity, although it was not possible to isolate individual antifungal compounds.

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