培养基和冷冻速率对衣原体冻干后存活的影响。

J J Theunissen, E Stolz, M F Michel
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引用次数: 16

摘要

观察悬浮介质和冷冻速率对冻干后沙眼衣原体LGV2和肺炎衣原体存活的影响。感染性初级体(EBs)的最大损失发生在冻干过程中。用含葡萄糖、蔗糖或乳糖的胎牛血清(FCS)作为冻干介质,其回收率(+/- 5%)高于用含蔗糖、谷氨酰胺和10% FCS (SPG)的蛋黄囊、脱脂乳或磷酸盐缓冲液(0.5-3%)。冻干后,在冷冻前10(4)至5 × 10(6)包涵体形成单位(ifu) ml-1的测试范围内,存活不受影响。在4℃下保存4个月后,回收的两种血清衣原体的ifu数量与冻干后立即恢复的ifu数量相同。结论:衣原体可以冻干形式储存和运输。然而,感染性EBs中95%的损失应该被考虑在内。
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The effects of medium and rate of freezing on the survival of chlamydias after lyophilization.

The effects of suspension media and rate of freezing on the survival of Chlamydia trachomatis LGV2 and Chlamydia pneumoniae after lyophilization were assessed. The highest loss in infectious elementary bodies (EBs) occurred during lyophilization. The survival was higher after freezing at a rate of 1 degree C min-1 and lyophilization than that after rapid freezing at -70 degrees C or -196 degrees C. The recovery (+/- 5%) was higher when fetal calf serum (FCS) containing glucose, saccharose or lactose were used as lyophilization media than that (0.5-3%) when yolk-sac, skimmed milk or phosphate buffer containing sucrose, glutamine and 10% FCS (SPG) were used. After lyophilization, the survival was not affected in the tested range from 10(4) to 5 x 10(6) inclusion-forming units (ifu) ml-1 prior to freezing. After storage for 4 months at 4 degrees C, the numbers of ifu of both Chlamydia serovars that were recovered were identical to the numbers of ifu immediately after lyophilization. It was concluded that chlamydias can be stored and transported in lyophilized form. However, a loss of 95% in infectious EBs should be taken into account.

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