尼日尔三角洲下游水生细菌生态学研究:标准平板计数生物的多重抗生素耐药性。

The Journal of applied bacteriology Pub Date : 1993-05-01
M T Ogan, D E Nwiika
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引用次数: 0

摘要

在河流州哈科特港地区研究了尼日尔三角洲下游淡水中多重抗生素耐药(MAR)细菌的生态学。在降低污染程度的基础上,划分为A、B、C三个区域。在最初的研究中,比较了两种活计数培养基,酪蛋白-蛋白胨-淀粉(CPS)和平板计数(PC)琼脂(含氯霉素、四环素、青霉素、链霉素或氨苄西林)的细胞回收率。从粪便污染的新卡拉巴尔河(A区)中,含有四环素、青霉素、链霉素和氨苄西林的CPS比含有类似抗生素的SPC琼脂检出更多的抗生素耐药(AR)细菌,但在森林溪流(B区)样品中则相反。在个别取样点也观察到两种介质之间的差异。在污染最轻的Isiokpo和Elele-Alimini溪流(C区)中,对氨苄西林耐药的AR细菌比例从55% (B区)到72% (C区)不等,而在每个区域中,对其他药物的耐药比例大多< 50%。在无抗生素的计数培养基上,从预防菌落型中纯化的30株细菌主要包括芽孢杆菌(12)和肠杆菌(18)。5 ~ 10株对>或= 3种抗生素耐药;其中7人对所有5种药物都有抗药性。大多数菌株的抗生素谱不同,取决于给药方法(贴片或琼脂掺入)、培养基和孵育温度(25℃、37℃或44.5℃),两种方法均有21株菌株对氨苄西林保持一致耐药;对氯霉素、四环素和青霉素有10 ~ 19例一致。(摘要删节250字)
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Studies on the ecology of aquatic bacteria of the lower Niger Delta: multiple antibiotic resistance among the standard plate count organisms.

The ecology of multiple antibiotic resistant (MAR) bacteria in the fresh-waters of the lower Niger Delta was studied in the Port Harcourt area, Rivers State. On the basis of decreasing pollution levels three zones, A, B, C, were recognized. Cell recovery by two viable count media, casein-peptone-starch (CPS) and plate count (PC) agar containing chloramphenicol, tetracycline, penicillin, streptomycin or ampicillin were compared in an initial study. Higher numbers of antibiotic resistant (AR) bacteria were recovered on CPS containing tetracycline, penicillin, streptomycin and ampicillin from the faecally-polluted New Calabar River (zone A) than on SPC agar containing similar antibiotics but the reverse was observed for forest stream (zone B) samples. Differences between the two media were also observed at individual sample sites. The proportions of strains of AR bacteria resistant to their primary isolation antibiotic varied from 55% (zone B) to 72% in the least polluted Isiokpo and Elele-Alimini streams (zone C), for ampicillin, and mostly < 50% for the other drugs in each zone. Thirty bacterial strains purified from the prevent colonial types on the count media without antibiotics included mainly species of Bacillus (12) and enterobacteria (18). Between five and 10 strains were resistant to > or = three antibiotics; seven were resistant to all five. The antibiograms of most strains were variable and depended on the method of drug application (discs or incorporation into agar), media and temperature of incubation (25 degrees, 37 degrees or 44.5 degrees C). Twenty-one strains were consistently resistant to ampicillin by the two methods; 10 to 19 were consistent for chloramphenicol, tetracycline and penicillin.(ABSTRACT TRUNCATED AT 250 WORDS)

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