产生环孢素的胀霉霉(ATCC 34921)独特的重复DNA序列

Frank Kempken, Claudia Schreiner, Kurt Schörgendorfer, Ulrich Kück
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引用次数: 15

摘要

Kempken, F., Schreiner, C., Schörgendorfer, K.和k ck, U. 1995。一个独特的重复DNA序列在产生环孢素的膨胀霉(ATCC 34921)。真菌学通报,2009,31(5):391 - 391。通过与真菌总DNA和rDNA探针的差异杂交,从产生环孢素a的真菌ATCC 34921中分离到含有重复DNA序列的重组λ克隆。从这个调查来看,1%的λ克隆似乎包含重复序列。随后的分析鉴定出了一个分散的重复DNA元件。它被命名为CPA元素(环孢素生产相关),似乎是菌株特异性的,因为它不存在于其他相关菌株或真菌中。与染色体限制片段杂交表明CPA元件在基因组中分布均匀。每个单倍体基因组的拷贝数估计在20到30之间。对CPA元件3个拷贝中0.9 kb的XhoI片段的序列分析表明,该序列在所有拷贝中都具有很强的保守性。一个200 bp的区域与玉米(Zea diploperennis)的重复序列相似。本文讨论了使用该DNA序列作为分子标记来鉴定这种产生环孢素的菌株ATCC 34921,并讨论了重复DNA序列与真菌核型重排的相关性。
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A Unique Repeated DNA Sequence in the Cyclosporin-Producing Strain of Tolypocladium inflatum (ATCC 34921)

Kempken, F., Schreiner, C., Schörgendorfer, K., and Kück, U. 1995. A unique repeated DNA sequence in the cyclosporin-producing strain of Tolypocladium inflatum (ATCC 34921). Experimental Mycology 19, 305-313. Recombinant λ clones containing repeated DNA sequences were isolated from the cyclosporin A-producing fungus Tolypocladium inflatum (ATCC 34921) by differential hybridization with total fungal DNA and rDNA probes. From this survey 1% of the λ clones appeared to contain repeated sequences. Subsequent analysis led to the identification of a dispersed repetitive DNA element. It was named CPA element (cyclosporin production associated) and appears to be strain specific, since it is absent from other related strains or fungi. Hybridization with chromosomal restriction fragments indicates an equal distribution of the CPA element in the genome. The copy number was estimated to be between 20 and 30 per haploid genome. Sequence analysis of a 0.9-kb XhoI fragment from three copies of the CPA element revealed strong conservation of this sequence among all copies. A 200-bp region exhibits similarities to a repeated sequence from Zea diploperennis. The use of this DNA sequence as a molecular marker for identification of this cyclosporin-producing strain ATCC 34921 is discussed as is the relevance of repeated DNA sequences for rearrangements of fungal karyotypes.

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