人巨细胞病毒DNA的检测:如何、何时、何地?

G Gerna, F Baldanti, D Zella, M Furione
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引用次数: 0

摘要

虽然已有几种方法用于检测和定量人巨细胞病毒(HCMV) DNA,但它们都可以分为三大类:(i)通过原位杂交或原位聚合酶链反应(PCR)直接检测组织中的HCMV DNA;(ii)通过与不同标记的DNA或RNA探针杂交来检测细胞或组织裂解物中的HCMV DNA -标记逐渐修改以提供更高的灵敏度(杂交产物通过放射性,比色或化学发光程序显示);(iii)通过定性(单步和巢式)和定量(半定量、竞争性或非竞争性)PCR检测细胞或组织裂解物中的HCMV DNA。所采用的方法的选择主要取决于必须评估的临床情况。HCMV基因组检测的临床样本必须有所不同。
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Detection of human cytomegalovirus DNA: how, when and where?

Although several methods have been utilized for the detection and quantification of human cytomegalovirus (HCMV) DNA, all of them can be divided into three groups: (i) detection of HCMV DNA directly in tissues by in situ hybridization or in situ polymerase chain reaction (PCR); (ii) detection of HCMV DNA in cell or tissue lysates by hybridization with DNA or RNA probes differently labelled-labels were progressively modified in order to provide an increasing sensitivity (hybridization products were revealed by radioactive, colorimetric or chemiluminescent procedures); (iii) detection of HCMV DNA in cell or tissue lysates by qualitative (single-step and nested) and quantitative (semiquantitative, competitive or noncompetitive) PCR. The selection of the methods to be employed depends primarily on the clinical situation which must be evaluated. Clinical samples for HCMV genome detection must vary accordingly.

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