{"title":"植物细胞碱性彗星试验:蚕豆根细胞DNA链断裂的一种新的遗传毒性试验","authors":"G. Koppen, L. Verschaeve","doi":"10.1016/S0165-1161(96)90017-5","DOIUrl":null,"url":null,"abstract":"<div><p>The alkaline comet assay (single cell gel electrophoresis assay) is a sensitive method for the detection of DNA damage. This paper describes the first application of this assay to plant cells for genotoxicological assessment. Germinating <em>Vicia faba</em> (field bean) seedlings were kept in water with either methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS), mitomycin C (MMC), cycloheximide (CH), cadmium chloride (CdCl<sub>2</sub>), potassium dichromate (K<sub>2</sub>Cr<sub>2</sub>O<sub>7</sub>), or chromium trichloride (CrCl<sub>3</sub>). Nuclei were isolated from the root cells and evaluated for the extent of DNA migration. With the exception of cycloheximide, all agents induced a significant increase in DNA migration. These results indicate that the comet assay may be a valuable tool for monitoring DNA damage in plant systems. However, there was a significant heterogeneity in the extent of DNA migration within and between seedlings, which may be intrinsic to the assay or indicative of sampling problems.</p></div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"360 3","pages":"Pages 193-200"},"PeriodicalIF":0.0000,"publicationDate":"1996-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90017-5","citationCount":"150","resultStr":"{\"title\":\"The alkaline comet test on plant cells: A new genotoxicity test for DNA strand breaks in Vicia faba root cells\",\"authors\":\"G. Koppen, L. Verschaeve\",\"doi\":\"10.1016/S0165-1161(96)90017-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The alkaline comet assay (single cell gel electrophoresis assay) is a sensitive method for the detection of DNA damage. This paper describes the first application of this assay to plant cells for genotoxicological assessment. Germinating <em>Vicia faba</em> (field bean) seedlings were kept in water with either methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS), mitomycin C (MMC), cycloheximide (CH), cadmium chloride (CdCl<sub>2</sub>), potassium dichromate (K<sub>2</sub>Cr<sub>2</sub>O<sub>7</sub>), or chromium trichloride (CrCl<sub>3</sub>). Nuclei were isolated from the root cells and evaluated for the extent of DNA migration. With the exception of cycloheximide, all agents induced a significant increase in DNA migration. These results indicate that the comet assay may be a valuable tool for monitoring DNA damage in plant systems. However, there was a significant heterogeneity in the extent of DNA migration within and between seedlings, which may be intrinsic to the assay or indicative of sampling problems.</p></div>\",\"PeriodicalId\":18870,\"journal\":{\"name\":\"Mutation Research\\\\/environmental Mutagenesis and Related Subjects\",\"volume\":\"360 3\",\"pages\":\"Pages 193-200\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-08-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90017-5\",\"citationCount\":\"150\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research\\\\/environmental Mutagenesis and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0165116196900175\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research\\/environmental Mutagenesis and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165116196900175","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The alkaline comet test on plant cells: A new genotoxicity test for DNA strand breaks in Vicia faba root cells
The alkaline comet assay (single cell gel electrophoresis assay) is a sensitive method for the detection of DNA damage. This paper describes the first application of this assay to plant cells for genotoxicological assessment. Germinating Vicia faba (field bean) seedlings were kept in water with either methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS), mitomycin C (MMC), cycloheximide (CH), cadmium chloride (CdCl2), potassium dichromate (K2Cr2O7), or chromium trichloride (CrCl3). Nuclei were isolated from the root cells and evaluated for the extent of DNA migration. With the exception of cycloheximide, all agents induced a significant increase in DNA migration. These results indicate that the comet assay may be a valuable tool for monitoring DNA damage in plant systems. However, there was a significant heterogeneity in the extent of DNA migration within and between seedlings, which may be intrinsic to the assay or indicative of sampling problems.
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