A Ohshige, M Ito, H Koyama, T Maeda, T Yoshimura, H Okamura
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引用次数: 0
摘要
目的:探讨雌激素和黄体酮对去卵巢大鼠血浆血小板活化因子乙酰水解酶(PAF-AH)活性的影响。方法:采用17 -乙炔雌醇(0.25 mg/kg;6天)或甲孕酮(50mg /kg;6天)。血浆PAF-AH活性的测定内和测定间变异系数分别为2.5%和4.4%。第5天用市售试剂盒测定血浆脂蛋白谱。结果:去卵巢大鼠在14天内血浆PAF-AH活性无明显变化。在去卵巢大鼠中，雌激素使PAF-AH活性降低高达85%，黄体酮使PAF-AH活性升高高达44%。血浆PAF-AH活性与高密度脂蛋白胆固醇呈正相关(r = 0.96, p < 0.001)。结论:雌激素和黄体酮可能通过高密度脂蛋白胆固醇对去卵巢大鼠血浆PAF-AH活性产生影响。
Effects of estrogen and progesterone on platelet-activating factor acetylhydrolase activity in ovariectomised rats.
Objective: To evaluate the effect of estrogen and progesterone on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity in ovariectomized rats.
Methods: Plasma PAF-AH activity was examined in ovariectomized rats treated with 17 alpha-ethynylestradiol (0.25 mg/kg; 6 days) or medroxyprogesterone (50 mg/kg; 6 days). The intra- and inter-assay coefficients of variation for plasma PAF-AH activity were 2.5% and 4.4%, respectively. Plasma lipoprotein profiles on day 5 were determined with commercially available kits.
Results: Ovariectomized rats showed no significant change in plasma PAF-AH activity over a 14 day period. Estrogen decreased the PAF-AH activity up to 85%, and progesterone increased PAF-AH activity up to 44% in ovariectomized rats. There was a positive correlation between plasma PAF-AH activity and high density lipoprotein cholesterol (r = 0.96, p < 0.001).
Conclusion: These data suggest that estrogen and progesterone exert an effect on plasma PAF-AH activity in ovariectomized rats, possibly via high density lipoprotein cholesterol.
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