MB-Dot-ELISA法检测脑膜炎奈瑟菌B群抗原。

Bulletin of the Pan American Health Organization Pub Date : 1996-09-01

G Alkmin M das, I M Landgraf, S H Shimizu

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引用次数: 0

摘要

脑膜炎奈瑟菌B群感染一直难以检测，部分原因是该细菌群的多糖是一种弱免疫原。本文描述了采用高滴度马抗血清检测脑膜炎奈瑟菌B群抗原的一种新方法(MB-Dot-ELISA)的工作。该研究分析了585名受试者的脑脊液样本，其中574名疑似脑膜炎病例和11名神经系统疾病患者。灵敏度为0.991，特异度为0.826。这些结果优于胶乳凝集法的结果，与反免疫电泳和细菌学方法的结果基本一致。总体而言，发现mb - dot酶联免疫吸附试验敏感、廉价，适合公共卫生实验室调查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Detection of N. meningitidis group B antigens by MB-Dot-ELISA in patients with meningitis.

Infection with Neisseria meningitidis group B has been difficult to detect, partly because this bacterial group's polysaccharide is a weak immunogen. This article describes work carried out to test a new procedure (MB-Dot-ELISA) employing a high-titered horse antiserum for detection of N. meningitidis group B antigens. The study assayed cerebrospinal fluid samples from 585 subjects, 574 with suspected meningitis cases and 11 with neurologic disorders. The results of the assay indicated a sensitivity of 0.991 and a specificity of 0.826. These results were superior to those obtained with latex agglutination and in substantial agreement with the results of counterimmunoelectrophoresis and bacteriologic methods. Overall, the MB-Dot-ELISA was found to be sensitive, inexpensive, and suitable for public health laboratory investigations.

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Bulletin of the Pan American Health Organization

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