Studies on promoting activity of Taiwan betel quid ingredients in hamster buccal pouch carcinogenesis

Previous studies indicated that Taiwan betel quid is a promoter rather than an initiator during the carcinogenesis of hamster buccal pouch carcinoma. The maximum promoting activity can be demonstrated 24 weeks after betel quid implantation, following an initial application of 0.5% DMBA (7,12-dimethyl benzanthracene) three times per week for 4 weeks. In the present study, components of Taiwan betel quid and its additives (dry betel nut fibre, piper betel, slaked lime, cold aqueous extract, and hot aqueous extract) were applied respectively or in various combinations to investigate their promoting activity. One hundred and thirty Syrian hamsters were divided into 13 groups based on different combinations of the betel quid ingredients applied. The incidence of tumours in the hamster buccal pouch was significantly higher in groups exposed to dry betel nut fibre (P < 0.01) and cold aqueous extract (P < 0.05). The results indicate that betel nut fibre and cold aqueous extract are the major components of betel quid that may promote carcinogenesis in the hamster buccal pouch.