猕猴肝细胞体外培养胞食疟原虫红细胞外期的研究。

Indian journal of malariology Pub Date : 1996-06-01
K K Kamboj, S K Puri, J C Katiyar, G P Dutta
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引用次数: 0

摘要

通过肝活检灌注获得的骨猴肝细胞,体外感染了在斯蒂芬按蚊中培养的食蟹性疟原虫巴斯泰纳利孢子子。在相差显微镜和吉姆萨染色下观察外红细胞(EE)分期的发展。孢子子接种后7-8天培养的肝细胞出现多核e期。
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In-vitro cultivation of exoerythrocytic stages of Plasmodium cynomolgi in hepatocytes of Macaca radiata.

Hepatocytes from bonet monkey (Macaca radiata) obtained by perfusion of a liver biopsy were infected in-vitro with Plasmodium cynomolgi bastianellii sporozoites raised in Anopheles stephensi. The development of exoerythrocytic (EE) stages was seen under phase contrast microscope and by Giemsa staining. Multinucleated EE-stages were seen in the cultured hepatocytes on day 7-8 post-sporozoite inoculation.

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