HUVEC中前列环素/血栓素A2的形成比例在随后的每一次传代中下降

Prostaglandins Pub Date : 1997-07-01 DOI:10.1016/S0090-6980(97)00063-4

K. Neubert, A. Haberland, I. Kruse, M. Wirth , I. Schimke

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引用次数: 23

摘要

在这项研究中，我们分析了不同传代人脐静脉内皮细胞(HUVEC)的抗氧化能力(SOD-、gsh - px -活性)和基础、H2O2-和atp刺激下PGI2和TXA2的形成。细胞的继代培养在一定程度上代表了衰老的过程。细胞继代培养和H2O2孵育对SOD和GSH-Px活性均无显著影响。H2O2 (0.1 mM和1.0 mM)对PGI2和TXA2的生成具有细胞传代时间依赖性。PGI2/TXA2的形成比从第1代的640:1 (0.1 mM H2O2)和430:1 (1.0 mM H2O2，孵育40 min)变为第4代的13:1和17:1。这是由于PGI2合成的减少与更明显的TXA2形成有关。在基础和atp刺激的类二十烷形成中发现了相同的行为。基于此，氧自由基形成的年龄依赖性激活可能是导致老年人类二十烷代谢改变导致血管并发症的原因。

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The Ratio of Formation of Prostacyclin/Thromboxane A2 in HUVEC Decreased in Each Subsequent Passage

In this study, we analyzed the antioxidative potential (SOD-, GSH-Px-activity) and the basal, H₂O₂- and ATP-stimulated formation of PGI₂ and TXA₂ in human umbilical vein endothelial cells (HUVEC) of different passages. The subcultivation of cells partly represents the process of aging.

Both subcultivation of the cells and the H₂O₂ incubation did not significantly influence the activity of SOD and GSH-Px.

H₂O₂ (0.1 mM and 1.0 mM) stimulated the generation of PGI₂ and TXA₂ in the cell passages time dependently. The formation ratio of PGI₂/TXA₂ changed from 640:1 (0.1 mM H₂O₂) or 430:1 (1.0 mM H₂O₂, 40 min incubation) at the 1st passage, to 13:1 and 17:1, respectively, at the 4th passage. This resulted from the reduction of the PGI₂ synthesis connected with more pronounced TXA₂ formation. The same behavior was found in the basal and ATP-stimulated eicosanoid formation.

Based on this, the age-dependent activation of the oxygen radical formation could be responsible for the modified eicosanoid metabolism resulting in vascular complications in the elderly.

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