Inge Panum Jensen , Ebbe Thisted , Graciela Glikmann , Niels Obel , Poul-Erik Kofoed , Manuel Sambo , Niels H. Valerius , Carl-Heinrich Mordhorst
{"title":"鼻咽吸出物中呼吸道合胞病毒分泌IgM和IgA抗体:抗原检测的诊断补充","authors":"Inge Panum Jensen , Ebbe Thisted , Graciela Glikmann , Niels Obel , Poul-Erik Kofoed , Manuel Sambo , Niels H. Valerius , Carl-Heinrich Mordhorst","doi":"10.1016/S0928-0197(97)10002-2","DOIUrl":null,"url":null,"abstract":"<div><p><strong>Background:</strong> RSV-shedding during an RSV-infection declines dramatically after the first week of infection. It could be of interest to be able to diagnose RSV-infection for a longer period of time by detection of specific RSV-IgM and RSV-IgA in nasopharyngeal aspirates (NPA) in order to minimize unnecessary antibiotics.</p><p><strong>Objectives:</strong> To evaluate an ELISA to detect specific RSV-IgM and RSV-IgA in NPA as a supplement to RSV-antigen detection.</p><p><strong>Study design:</strong> A total of 104 NPA from 101 children (median age 9 months) with acute respiratory disease (group 1) admitted to hospital and consecutive NPA (collected on day 0, 7, 14, 30 and 60) from 11 children (median age 3 months) with a proven RSV infection (group 2) were collected. All NPA from group 1 were analysed for RSV-antigen, RSV-IgM and RSV-IgA. NPA from group 2 were analysed for RSV-IgM and RSV-IgA.</p><p><strong>Results:</strong> Thirty-five NPA in group 1 were positive for RSV-antigen and 64 were positive for RSV-IgM. When ‘true’ RSV infection was defined by the detection of RSV-antigen and/or RSV-IgM the RSV-antigen test alone found 44% and the RSV-IgM test alone found 80%. In group 2 <span><math><mtext>8</mtext><mtext>11</mtext></math></span> (73%) had an excellent RSV-IgM response day 7, the rest responded later. Only <span><math><mtext>5</mtext><mtext>11</mtext></math></span> (46%) had a less pronounced RSV-IgA response on day 7, three cases responded later and three did not respond at all. RSV-IgM disappeared in <span><math><mtext>8</mtext><mtext>11</mtext></math></span> (73%) and RSV-IgA in <span><math><mtext>7</mtext><mtext>8</mtext></math></span> (88%) between day 30–60.</p><p><strong>Conclusions:</strong> Specific RSV-IgM is a valuable supplement to RSV-antigen detection for the diagnosis of acute and recent RSV infection.</p></div>","PeriodicalId":79479,"journal":{"name":"Clinical and diagnostic virology","volume":"8 3","pages":"Pages 219-226"},"PeriodicalIF":0.0000,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-0197(97)10002-2","citationCount":"13","resultStr":"{\"title\":\"Secretory IgM and IgA antibodies to respiratory syncytial virus in nasopharyngeal aspirates: a diagnostic supplement to antigen detection\",\"authors\":\"Inge Panum Jensen , Ebbe Thisted , Graciela Glikmann , Niels Obel , Poul-Erik Kofoed , Manuel Sambo , Niels H. Valerius , Carl-Heinrich Mordhorst\",\"doi\":\"10.1016/S0928-0197(97)10002-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><strong>Background:</strong> RSV-shedding during an RSV-infection declines dramatically after the first week of infection. It could be of interest to be able to diagnose RSV-infection for a longer period of time by detection of specific RSV-IgM and RSV-IgA in nasopharyngeal aspirates (NPA) in order to minimize unnecessary antibiotics.</p><p><strong>Objectives:</strong> To evaluate an ELISA to detect specific RSV-IgM and RSV-IgA in NPA as a supplement to RSV-antigen detection.</p><p><strong>Study design:</strong> A total of 104 NPA from 101 children (median age 9 months) with acute respiratory disease (group 1) admitted to hospital and consecutive NPA (collected on day 0, 7, 14, 30 and 60) from 11 children (median age 3 months) with a proven RSV infection (group 2) were collected. All NPA from group 1 were analysed for RSV-antigen, RSV-IgM and RSV-IgA. NPA from group 2 were analysed for RSV-IgM and RSV-IgA.</p><p><strong>Results:</strong> Thirty-five NPA in group 1 were positive for RSV-antigen and 64 were positive for RSV-IgM. When ‘true’ RSV infection was defined by the detection of RSV-antigen and/or RSV-IgM the RSV-antigen test alone found 44% and the RSV-IgM test alone found 80%. In group 2 <span><math><mtext>8</mtext><mtext>11</mtext></math></span> (73%) had an excellent RSV-IgM response day 7, the rest responded later. Only <span><math><mtext>5</mtext><mtext>11</mtext></math></span> (46%) had a less pronounced RSV-IgA response on day 7, three cases responded later and three did not respond at all. RSV-IgM disappeared in <span><math><mtext>8</mtext><mtext>11</mtext></math></span> (73%) and RSV-IgA in <span><math><mtext>7</mtext><mtext>8</mtext></math></span> (88%) between day 30–60.</p><p><strong>Conclusions:</strong> Specific RSV-IgM is a valuable supplement to RSV-antigen detection for the diagnosis of acute and recent RSV infection.</p></div>\",\"PeriodicalId\":79479,\"journal\":{\"name\":\"Clinical and diagnostic virology\",\"volume\":\"8 3\",\"pages\":\"Pages 219-226\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0928-0197(97)10002-2\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and diagnostic virology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0928019797100022\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and diagnostic virology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928019797100022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Secretory IgM and IgA antibodies to respiratory syncytial virus in nasopharyngeal aspirates: a diagnostic supplement to antigen detection
Background: RSV-shedding during an RSV-infection declines dramatically after the first week of infection. It could be of interest to be able to diagnose RSV-infection for a longer period of time by detection of specific RSV-IgM and RSV-IgA in nasopharyngeal aspirates (NPA) in order to minimize unnecessary antibiotics.
Objectives: To evaluate an ELISA to detect specific RSV-IgM and RSV-IgA in NPA as a supplement to RSV-antigen detection.
Study design: A total of 104 NPA from 101 children (median age 9 months) with acute respiratory disease (group 1) admitted to hospital and consecutive NPA (collected on day 0, 7, 14, 30 and 60) from 11 children (median age 3 months) with a proven RSV infection (group 2) were collected. All NPA from group 1 were analysed for RSV-antigen, RSV-IgM and RSV-IgA. NPA from group 2 were analysed for RSV-IgM and RSV-IgA.
Results: Thirty-five NPA in group 1 were positive for RSV-antigen and 64 were positive for RSV-IgM. When ‘true’ RSV infection was defined by the detection of RSV-antigen and/or RSV-IgM the RSV-antigen test alone found 44% and the RSV-IgM test alone found 80%. In group 2 (73%) had an excellent RSV-IgM response day 7, the rest responded later. Only (46%) had a less pronounced RSV-IgA response on day 7, three cases responded later and three did not respond at all. RSV-IgM disappeared in (73%) and RSV-IgA in (88%) between day 30–60.
Conclusions: Specific RSV-IgM is a valuable supplement to RSV-antigen detection for the diagnosis of acute and recent RSV infection.
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