2g离心对大鼠长骨形的影响。

L Vico, O Barou, N Laroche, C Alexandre, M H Lafage-Proust
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引用次数: 28

摘要

超重力可以被认为是抵消微重力对骨组织有害影响的一种手段。用组织形态学法研究了离心提供的4天超重力暴露对骨组织的影响。将53日龄雄性sd大鼠随机分为离心组(2g, n = 10)、旋转组(ROTATE, n = 6)和静止对照组(control, n = 10)。旋转组为将1.03 g置于离心机旋转中心附近的笼子中。与对照组相比,2g大鼠的体重减少了16%。胫骨生长板宽度减小2g。2 / 10的2g大鼠肥厚带受损。在胫骨和肱骨初级海绵层(1度)中,分别减少1度海绵层宽度(-35%和-24%,分别旋转与控制;-37%和-41%,分别为2g)与骨增重相关(胫骨和肱骨旋转与对照组相比增加27%;+ 16%和+20%,2g分别与CONTROL相比),在ROTATE和2g中均观察到。在胫骨继发性(2度)海绵中,由于小梁较厚,2g大鼠的骨量增加(比对照组增加13% 2g),但由于小梁较薄,ROTATE大鼠的骨量减少(比对照组减少12%)。2g组和ROTATE组刺激了形成和吸收活性参数,2g组的形成活性增强更明显。两组肱骨2度海绵均未见结构改变。与CONTROL相比,2g和ROTATE组骨形成参数降低,但与CONTROL相比,2g组骨吸收活性增加,ROTATE组骨吸收活性降低。综上所述,早在第4天,2g超重力就导致软骨内骨形成减少,松质骨量增加。旋转导致不同的结果,包括软骨内骨形成减少,1度海绵状椎体骨体积增加,2度海绵状椎体骨丢失。
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Effects of centrifuging at 2g on rat long bone metaphyses.

Hypergravity may be considered as a means of counteracting the deleterious effects of microgravity on bone tissue. The effects of exposure to 4 days of hypergravity provided by centrifuging, on bone tissue were studied using histomorphometry. Young 53-day-old male Sprague Dawley rats were randomly divided into a centrifuged group (2g, n = 10), a rotated group (ROTATE, n = 6) of rats exposed to 1.03 g placed in cages near the centre of rotation of the centrifuge and a stationary control group (CONTROL, n = 10). The body mass of the 2g rats was decreased by this experience by 16% compared to CONTROL. The width of the tibial growth plate of 2g was decreased. In two out of ten 2g rats, the hypertrophic zone was injured. In both the tibial and humeral primary (1 degrees ) spongiosae, a reduced 1 degrees spongiosa width (-35% and -24%, ROTATE versus CONTROL respectively; -37% and -41%, 2g versus CONTROL respectively) associated with bone gain (+27% for tibia and humerus ROTATE versus CONTROL; + 16% and +20%, 2g versus CONTROL respectively) was observed in both ROTATE and 2g. In the tibial secondary (2 degrees) spongiosa, bone mass was increased in the 2g (+13% 2g versus CONTROL) rats due to thicker trabeculae, but was decreased in ROTATE rats (-12% versus CONTROL) due to thinner trabeculae. The parameters of formation and resorption activities were stimulated in the 2g and ROTATE groups, the formation activity being more enhanced in 2g. No structural changes were observed in the humeral 2 degrees spongiosa in any of the groups. Numeral bone formation parameters were decreased in 2g and ROTATE but resorption activity was increased in 2g and decreased in ROTATE compared to CONTROL. In conclusion, as early as the 4th day, 2g hypergravity induced reduced endochondral bone formation and increased cancellous bone mass. Rotation led to mixed results including reduced endochondral bone formation, increased bone volume in the 1 degrees spongiosa and bone loss in the 2 degrees spongiosa.

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Acknowledgement to referees Acknowledgement to referees Acknowledgement to referees Acknowledgement to referees In vivo vibrational wave propagation in human tibiae at different ages
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