巢式聚合酶链反应鉴定恙虫立克次体血清型。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1996-05-01

G J Shieh, H L Chen, H Y Chen, G R Wang, C B Horng

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引用次数: 0

摘要

采用巢式聚合酶链反应(巢式PCR)对恙虫病立克次体进行血清型鉴定。PCR引物基于编码立克次体56 kDa抗原的核苷酸序列。与传统的免疫荧光法(IFA)在不同物种间表现出相当程度的交叉反应性相比，结果表明聚合酶链反应法比IFA更可靠。

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Identification of serotypes of Rickettsia tsutsugamushi by nested polymerase chain reaction.

Nested polymerase chain reaction (PCR) was applied to identify the serotypes of Rickettsia tsutsugamushi isolated from patients. The primers used for PCR were based on the nucleotide sequences encoding a 56 kDa antigen of rickettsiae. Comparing to the conventional immunofluorescence assay (IFA), which displays a considerable degree of cross-reactivity among different species, the result obtained suggests that the polymerase chain reaction method is much more reliable than IFA.

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Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology

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