正常、sv40转化的人角质形成细胞和癌细胞的荧光光谱。

Cancer biochemistry biophysics Pub Date : 1999-07-01

A J Papadopoulos, N N Zhadin, M L Steinberg, R R Alfano

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引用次数: 0

摘要

测定并比较SV40感染的人角质形成细胞、A431和SCC324癌细胞与正常人角质形成细胞的天然荧光发射光谱和激发光谱。在正常细胞和癌细胞或病毒转化细胞之间发现了细胞内NADH代谢状态的差异。观察到的差异，即癌症细胞和病毒感染细胞中结合的线粒体NADH比例增加，表现为发射光谱中的蓝色光谱偏移。

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Fluorescence spectroscopy of normal, SV40-transformed human keratinocytes, and carcinoma cells.

Native fluorescence emission and excitation spectra of SV40 infected human keratinocytes, A431 and SCC324 carcinoma cells, and normal human keratinocytes were measured and compared. A difference in the intracellular metabolic state of NADH was found between the normal cells and the cancer or virus-transformed cells. The observed difference, namely an increased proportion of bound, mitochondrial NADH in the cancer and virus-infected cells, manifests as a blue spectral shift in the emission spectra.

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Cancer biochemistry biophysics

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