荧光原位杂交技术在鸡染色体上定位ALDH、BMP-2、R-FABP、ifn - γ、rxr - γ和VIM基因。

M Guttenbach, I Nanda, P M Brickell, R Godbout, P Staeheli, Z E Zehner, M Schmid
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引用次数: 14

摘要

以基因组克隆和cDNA克隆为探针,采用荧光原位杂交技术(FISH)在鸡中定位了编码ALDH、BMP-2、R-FABP、ifn - γ、rxr - γ和VIM的6个结构基因。这些基因被发现位于4条不同的大染色体上:1号染色体(IFNG和FABP)、2号染色体(VIM和ALDH)、3号染色体(BMP2)和一条较小的大染色体,最有可能是7号染色体(RXRG)。除了IFNG外,所有新定位的位点都不对应于鸡和人类染色体之间已知的同源区域。
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Chromosomal localization of the genes encoding ALDH, BMP-2, R-FABP, IFN-gamma, RXR-gamma, and VIM in chicken by fluorescence in situ hybridization.

Six structural genes encoding ALDH, BMP-2, R-FABP, IFN-gamma, RXR-gamma and VIM were mapped in the chicken by fluorescence in situ hybridization (FISH) using genomic and cDNA clones as probes. The genes were found to be located on four different macrochromosomes: chromosome 1 (IFNG and FABP), chromosome 2 (VIM and ALDH), chromosome 3 (BMP2) and a smaller macrochromosome, most probably chromosome 7 (RXRG). With the exception of IFNG none of the newly mapped sites corresponds to known orthologous regions between chicken and human chromosomes.

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