{"title":"不同胁迫条件下黑曲霉过氧化氢酶的生产。","authors":"J Fiedurek","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The conidia of Aspergillus niger were cultured under various stress conditions. The highest yield of catalase activity was obtained after addition 1.2 M NaCl to 6-h-old culture, which improved the activity of the enzyme by about 2.8-fold in comparison with the control medium without this depressor. A significantly lower increase (1.3- to 1.7-fold) in catalase activity was reached when spores were incubated under oxidative stress (100-200 mM H2O2) or at low pH (2 and 3). Respiratory activities in A. niger culture show important changes with the osmotic stress increase.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"49 1","pages":"43-9"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Production of Aspergillus niger catalase under various stress conditions.\",\"authors\":\"J Fiedurek\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The conidia of Aspergillus niger were cultured under various stress conditions. The highest yield of catalase activity was obtained after addition 1.2 M NaCl to 6-h-old culture, which improved the activity of the enzyme by about 2.8-fold in comparison with the control medium without this depressor. A significantly lower increase (1.3- to 1.7-fold) in catalase activity was reached when spores were incubated under oxidative stress (100-200 mM H2O2) or at low pH (2 and 3). Respiratory activities in A. niger culture show important changes with the osmotic stress increase.</p>\",\"PeriodicalId\":75388,\"journal\":{\"name\":\"Acta microbiologica Polonica\",\"volume\":\"49 1\",\"pages\":\"43-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta microbiologica Polonica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Polonica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
在不同的胁迫条件下培养黑曲霉的分生孢子。在6 h的培养基中添加1.2 M NaCl后,过氧化氢酶活性最高,比不添加该抑制剂的对照培养基提高了约2.8倍。在氧化胁迫(100-200 mM H2O2)或低pH(2和3)条件下培养孢子时,过氧化氢酶活性的增加幅度较低(1.3- 1.7倍)。黑曲霉培养的呼吸活性随着渗透胁迫的增加而发生重要变化。
Production of Aspergillus niger catalase under various stress conditions.
The conidia of Aspergillus niger were cultured under various stress conditions. The highest yield of catalase activity was obtained after addition 1.2 M NaCl to 6-h-old culture, which improved the activity of the enzyme by about 2.8-fold in comparison with the control medium without this depressor. A significantly lower increase (1.3- to 1.7-fold) in catalase activity was reached when spores were incubated under oxidative stress (100-200 mM H2O2) or at low pH (2 and 3). Respiratory activities in A. niger culture show important changes with the osmotic stress increase.